Unlock instant, AI-driven research and patent intelligence for your innovation.

Decellularized tongue matrix material and preparation method thereof

A matrix material and decellularization technology, applied in the field of acellular tongue matrix material and its preparation, can solve the problems of incomplete application and the like, and achieve the effects of simple method, good mechanical properties and good adhesion properties.

Active Publication Date: 2015-04-08
SUN YAT SEN UNIV
View PDF7 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of glucopyranoside reduces the potential biological toxicity of the detergent to the replanted cells during the cleaning process, it is mainly applied to tissues and organs containing more collagen fibers and elastic fibers, and it is applied to abundant and well-developed muscle fibers. Not fully applicable in the tongue tissue

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Decellularized tongue matrix material and preparation method thereof
  • Decellularized tongue matrix material and preparation method thereof
  • Decellularized tongue matrix material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Production of Mouse Tongue ECM

[0048] (1) Material collection: the tongue tissues of healthy mice were selected, and both sides were washed with PBS to remove blood stains. As for the EP tube, store it in a -80°C refrigerator for later use.

[0049](2) Broken cells: Take out the tissue block from the -80°C refrigerator, thaw it in a 37°C water bath, and then put it back into the -80°C refrigerator for 30 minutes, and repeat the freezing and thawing process 3 times.

[0050] (3) Disinfection and preparation: Place the tissue block in 75% alcohol, suspend the tongue tissue block with sutures, hang it in a 250ml jar, and place a 1cm magnetic stirring bar at the bottom of the bottle.

[0051] (4) Pre-cleaning and crushing: add 250ml of sterile third-grade water, and treat on a magnetic stirrer (gear 2) at 4°C for 12 hours.

[0052] (5) Cleaning of cell fragments: the tongue tissue is changed to a small-scale stirring circulator equipped with 250ml of 1M NaCl s...

Embodiment 2

[0065] Embodiment 2, the making of pig tongue ECM

[0066] (1) Material collection: select healthy pig tongue tissue, decompose it into small pieces of one centimeter square, and wash both sides with PBS to remove blood stains. As for the EP tube, store it in a -80°C refrigerator for later use.

[0067] (2) Broken cells: Take out the tissue block from the -80°C refrigerator, thaw it in a 37°C water bath, and then put it back into the -80°C refrigerator for 30 minutes, and repeat the freezing and thawing process 3 times.

[0068] (3) Disinfection and preparation: Place the tissue block in 75% alcohol, suspend the tongue tissue block with sutures, hang it in a 250ml jar, and place a 1cm magnetic stirring bar at the bottom of the bottle.

[0069] (4) Pre-cleaning and crushing: add 250ml of sterile third-grade water, and treat on a magnetic stirrer (gear 2) at 4°C for 12 hours.

[0070] (5) Cleaning of cell fragments: the tongue tissue was replaced in a small stirring circulator...

Embodiment 3

[0079] Embodiment 3, the making of rat tongue ECM

[0080] (1) Material collection: select the tongue tissue of a healthy rat, decompose it into small pieces with a square of one centimeter, wash both sides with PBS to remove blood stains. As for the EP tube, store it in a -80°C refrigerator for later use.

[0081] (2) Broken cells: Take out the tissue block from the -80°C refrigerator, thaw it in a 37°C water bath, and then put it back into the -80°C refrigerator for 30 minutes, and repeat the freezing and thawing process 3 times.

[0082] (3) Disinfection and preparation: Place the tissue block in 70% alcohol, suspend the tongue tissue block with sutures, hang it in a 250ml jar, and place a 1cm magnetic stirring bar at the bottom of the bottle.

[0083] (4) Pre-cleaning and crushing: add 250ml of sterile third-grade water, and treat on a magnetic stirrer (gear 2) at 4°C for 12 hours.

[0084] (5) Cleaning of cell fragments: the tongue tissue was replaced in a small stirrin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a decellularized tongue matrix material and a preparation method thereof. The decellularized tongue matrix material is characterized in that tongue tissues of healthy animals are selected for physical, chemical and enzyme comprehensive method processing; lower osmatic solution, high osmatic solution, Triton X-100 and nuclease low-temperature processing is carried out to prepare the decellularized tongue matrix material; and the decellularized tongue matrix material with the good biocompatibility and the appropriate biodegradation rate is obtained. The material can be used for tongue regeneration and repairing and tongue disease studying models. The material is wide in source, easy to obtain, low in price and easy to manufacture.

Description

technical field [0001] The invention belongs to the field of processing biomedical materials, and in particular relates to a decellularized tongue matrix material and a preparation method thereof. Background technique [0002] The extracellular matrix (ECM) is a protein and polysaccharide macromolecular substance secreted by cells into the extracellular mesenchyme. ECM constitutes a complex grid connection tissue structure through a highly organized extracellular microenvironment, and plays a key role in regulating cell physiological activities such as cell survival and differentiation. Therefore, scaffold substrates and scaffold materials that can mimic the ECM are ideal carriers for tissue engineering research and tissue repair. The contact between cells and ECM is dynamic, and changes in ECM will lead to changes in the morphology and function of neighboring cells. An ideal scaffold material for tissue engineering needs to provide the same or similar microenvironment (in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
Inventor 张雁赵龙余姝毅郑骏恒
Owner SUN YAT SEN UNIV