Centrifugal chip for quickly detecting hepatitis B, hepatitis C and syphilis in blood and detection method

A blood and hepatitis C technology, applied in chemical instruments and methods, measuring devices, instruments, etc., can solve problems such as the limitations of the industrialization development of microfluidic chips, and achieve accelerated antigen-antibody reaction, shortened reaction time, and increased specific surface area. Effect

Active Publication Date: 2015-04-08
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it needs a large number of complex valve pump structure designs and external syringe pumps t

Method used

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  • Centrifugal chip for quickly detecting hepatitis B, hepatitis C and syphilis in blood and detection method
  • Centrifugal chip for quickly detecting hepatitis B, hepatitis C and syphilis in blood and detection method
  • Centrifugal chip for quickly detecting hepatitis B, hepatitis C and syphilis in blood and detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0051] see Figure 1 to Figure 3 , the device structure of the present invention is as follows:

[0052] The body is in the shape of a disc ( figure 1 and figure 2 Only 1 / 4 is shown in the figure), and there are 4 fan-shaped microfluidic unit modules evenly on each disc. The microfluidic module has a three-layer structure, including a base sheet A, a base sheet B, and a cover sheet C. Sealed alignment bonding can be achieved between the three layers.

[0053] Channel structure see figure 1 , each microfluidic unit module includes: a first washing liquid pool 1, a second washing liquid pool 2, a reaction pool 3, an enzyme liquid pool 5, a waste liquid pool 8, two detection liquid pools 6 and a stop liquid pool7. in:

[0054] The waste liquid pool 8 is arranged near the edge of the optical disk, and the third channel 3-1 with an aperture of 800 μm is arranged between the reaction pool 3 and the waste liquid pool 8, and the channel 3-1 is arranged on the side of the reacti...

Embodiment 2

[0063] Design and manufacture a centrifugal microfluidic chip for rapid blood detection, using PMMA as the chip material, and using a laser marking machine to process the channel structure. Similar to Example 1, a three-layer structure of the bottom sheet, the base sheet and the cover sheet is adopted, and through alignment and bonding, a certain space is created for the storage of liquids and samples. Pack the microbeads and reagents into the chip, and align and bond with the cover slip. Place the bonded centrifugal microfluidic chip on the centrifugal drive device, clamp the slot and tighten the rotor. Inject 100ul hepatitis B antigen standard substance into the liquid inlet with a micro syringe, the standard concentration is 0.2IU / ml, 0.4IU / ml, 2IU / ml, 4IU / ml respectively, the liquid enters the reaction pool and incubates for 10 minutes. Then start the centrifuge and centrifuge at a speed of 200 rpm, and the blood in the reaction pool 3 flows into the waste liquid pool. A...

Embodiment 3

[0065] Chip design and processing are the same as in Example 1, and the operation process is the same as in Example 2. Add hepatitis B antigen standard substances with concentrations of 0.2IU / ml, 0.5IU / ml, 2IU / ml, 3IU / ml, and 4IU / ml to the chip for reaction. After the reaction, measure the absorbance with a microplate reader and repeat 3 times. The result is as Figure 5 shown.

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Abstract

The invention discloses a centrifugal chip for quickly detecting hepatitis B, hepatitis C and syphilis in blood and a detection method. The chip is characterized in that main bodies are in disc shapes and at least four microfluidic unit modules are uniformly distributed on each main body; each microfluidic unit module comprises a first lotion pool (1), a second lotion pool (2), a reaction pool (3), an enzyme solution pool (5), a waste liquid pool (8), two detection liquid pools (6) and a terminating liquid pool (7). The detection method comprises the following steps: injecting a detection liquid into each unit module in advance during detection; then adding a sample; and centrifugalizing in the levels. The chip and detection method disclosed by the invention can be used for quickly improving the detection efficiency.

Description

technical field [0001] The field that the present invention relates to is a microfluidic chip and its application field. In particular, it relates to a centrifugal force-driven microfluidic chip device for rapid detection of hepatitis B, hepatitis C and syphilis in blood and a method for its use. Background technique [0002] Blood safety is related to human health, and it is of great significance to blood detection. The traditional blood test is an analysis method based on antigen-antibody specific combination, which has extremely high sensitivity and specificity, but the incubation time of conventional immunoassay is long, and it cannot be detected quickly in real time. At the same time, the operation process is cumbersome and consumes a lot of reagents and samples , and the detection equipment is relatively large, and the requirements for the experimental site are relatively high, so it is difficult to meet the requirements of on-site inspection. Therefore, it is of gre...

Claims

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Application Information

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IPC IPC(8): G01N33/576G01N33/569B01L3/00
CPCB01L3/50273B01L2300/0806B01L2400/0409G01N33/571G01N33/5761G01N33/5767
Inventor 郭嘉戴彰周勇亮张忠英
Owner XIAMEN UNIV
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