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Antibacterial peptide VIP (vasoactive intestinal peptide)-like peptide and preparation method thereof

A technology of antimicrobial peptides and boxes, applied in the field of bioengineering, can solve problems such as complex structure, insufficient antibacterial activity, and low antibacterial spectrum, and achieve the effects of simple production process, strong antibacterial ability, and wide antibacterial spectrum

Inactive Publication Date: 2015-04-22
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of antimicrobial peptide VIP similar peptide and its preparation method, to solve the limitations of complex structure, insufficient antibacterial activity and not wide antibacterial spectrum existing in the prior art

Method used

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  • Antibacterial peptide VIP (vasoactive intestinal peptide)-like peptide and preparation method thereof
  • Antibacterial peptide VIP (vasoactive intestinal peptide)-like peptide and preparation method thereof
  • Antibacterial peptide VIP (vasoactive intestinal peptide)-like peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Preparation method of antibacterial peptide VIP-like peptide:

[0042] (1) Synthesis of antimicrobial peptide VIP-like peptide gene:

[0043] Natural animal-derived antibacterial peptide is a neuroimmunomodulatory peptide with a simple and clear structure, a variety of biological activities, and a small concentration, which has development potential; but its antibacterial activity is relatively low. On the basis of its safety, the expired antibacterial activity is improved, and it is prepared by genetic engineering to improve its applicability.

[0044] According to the amino acid sequence, structural characteristics and physicochemical properties of the antimicrobial peptide VIP in GeneBank, D3 and D8 were replaced with K3 and K8 to improve them. The amino acid sequence before and after the improvement is as follows:

[0045] Before improvement: HSDAVFTDNYTRLRKQMAVKKYLNSILN

[0046] After improvement: HSKAVFTKNYTRLRKQMAVKKYLNSILN

[0047] In order to avoid the toxici...

Embodiment 2

[0065] Example 2 Determination of the antibacterial activity of the antibacterial peptide VIP-like peptide:

[0066] Use the Oxford cup method to determine the antibacterial activity of the purified antibacterial peptide VIP-like peptide, and cultivate E. coli ATCC 25922 to OD 600 About 0.5, prepare 100 mL of MHB medium containing 1.5% agar, cool the sterilized MH medium to about 50°C, add 1 mL of the above bacterial solution, mix well and pour the plate. After the medium is solidified, put an Oxford cup vertically on the surface of the medium, and add the test sample to the Oxford cup, including recombinantly expressed antibacterial peptide VIP-like peptide (enterokinase digestion fusion protein solution) and chemically synthesized antibacterial peptide VIP Incubate at 37°C for 16-18h. In the Oxford Cup antibacterial test, the antibacterial substance spreads on a spherical surface. The closer to the cup, the greater the concentration of antibacterial substances, and the farther ...

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Abstract

The invention discloses an antibacterial peptide VIP (vasoactive intestinal peptide)-like peptide. The antibacterial peptide VIP-like peptide is shown in the following amino acid sequence: HSKAVFTKNYTRLRKQMAVKKYLNSILN, and the invention also provides a preparation method of the antibacterial peptide VIP-like peptide. The antibacterial peptide VIP-like peptide has the advantages that a production technology is simple, production cost is low, antimicrobial activity is strong, antibacterial spectrum is broad, market prospect is broad, and the defects of complex structure, low antibacterial activity and narrow antibacterial spectrum in the prior art are overcome.

Description

Technical field [0001] The invention belongs to the technical field of bioengineering, and relates to an antibacterial peptide VIP-like peptide and a preparation method thereof. Background technique [0002] Harmonious animal husbandry is the theme of my country's current animal husbandry development. One of its important contents is the animal-human-nature harmony, and animal health is directly related to the quality and safety of animal-derived food and the environment. However, the massive use and abuse of antibiotics have led to the production of resistant strains of pathogenic bacteria and even super bacteria, the residue of antibiotics in livestock and poultry products, and the pollution of the environment with animal feces and urine, which seriously affect the quality of livestock products and human health. Therefore, it is extremely urgent to develop safe, efficient and environmentally friendly disease-prevention and health-care growth-promoting additives to replace antib...

Claims

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Application Information

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IPC IPC(8): C07K14/575C12N15/16C12N15/70
CPCC07K14/575C12N15/70
Inventor 徐春兰乔想金孟尧陈凯高紫阳牛卫宁尚晓娅
Owner NORTHWESTERN POLYTECHNICAL UNIV
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