Sanqingshan cold-resistant dendrobium officinale seedling culturing method

A technology of Dendrobium officinale and a cultivation method, which is applied in horticultural methods, botanical equipment and methods, plant genetic improvement and other directions, can solve problems such as no public reports, and achieves rich germplasm resources, stable cold resistance, high content of effective components, The effect of speeding up selection

Inactive Publication Date: 2015-04-29
江西瀚野生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, scientific research units and enterprises in Zhejiang, Guangdong, Yunnan and other provinces have carried out artificial breeding research on Dendrobium officinale, and have bred Xiandrobium No. 1, Xiandou No. 2, Zhongke No. 1, Hongxin No. 1, etc. New varieties (lines), these new varieties (lines) are all full-sibling or half-sibling families, that is, after selfing or hybridization of specific new plants, and then using their capsules (seeds) to reproduce to form the next generation of seedling populations, and The main purpose of breeding these new varieties (lines) is high yield and high quality, and there is no public report on the research on resistance

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0048]A method for cultivating cold-resistant dendrobium officinale seedlings in Mount Sanqingshan, the specific steps are as follows:

[0049] I: Hybrid breeding of Dendrobium officinale

[0050] i. Collect 30 germplasms of wild Dendrobium officinale in Mount Sanqingshan, and 30 germplasms of Huoshan rice dendrobium;

[0051] ii. With the strong plants of wild Dendrobium officinale in Sanqingshan as the female parent, and the strong plants of Huoshan rice dendrobium as the male parent, hybridization was carried out to obtain capsules. In the examples, the germplasm of Dendrobium candidum collected was cultivated, and compared among plants, the selected Plants with good overall performance and high content of active ingredients were used as new female parents and male parents for hybridization, and the selection criteria for overall better performance were: plant height, stem shape and thickness, color of internode longitudinal stripes, and leaf color This type of appearance ...

Embodiment 1

[0063] The active ingredient content data of the cold-resistant Dendrobium candidum obtained in Example 1 (5 strains are randomly selected in each area).

[0064] Table 1 Content data of active ingredients in cold-resistant Dendrobium officinale

[0065]

[0066] As can be seen from Table 1, the cold-resistant Dendrobium officinale cultivated by the method of the present invention has a high active ingredient content, and is a high-quality Dendrobium officinale.

Embodiment 2

[0068] A method for cultivating cold-resistant dendrobium officinale seedlings in Mount Sanqingshan, the specific steps are as follows:

[0069] I: Hybrid breeding of Dendrobium officinale

[0070] i. Collect 30 germplasms of wild Dendrobium officinale in Mount Sanqingshan, and 30 germplasms of Huoshan rice dendrobium;

[0071] ii. With the strong plants of wild Dendrobium officinale in Sanqingshan as the female parent, and the strong plants of Huoshan rice dendrobium as the male parent, hybridization was carried out to obtain capsules. In the examples, the germplasm of Dendrobium candidum collected was cultivated, and compared among plants, the selected Plants with better overall performance and high content of active ingredients were used as new plants for hybridization, and the selection criteria for better overall performance were: appearance indicators such as plant height, stem shape and thickness, internode longitudinal stripe color, and leaf color; The standard of act...

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Abstract

The invention discloses a sanqingshan cold-resistant dendrobium officinale seedling culturing method. The culturing method sequentially comprises the following steps: hybridizing and breeding dendrobium officinale, improving a dendrobium officinale population, breeding high-quality dendrobium officinale variety, and carrying out expanding propagation on high-quality dendrobium officinale seedlings. The group improvement is combined with breeding of high-quality individual plant, the breeding of a high-quality new variety strain and fast propagation of high-quality seedlings by applying plant tissue cell culture technology through adopting the progeny obtained from hybridization of sanqingshan wild dendrobium officinale and dendrobium huoshanense, varieties can be screened out according to cold resistance and content of active ingredients by utilizing low-temperature induction, and the breeding speed of the new strain can be greatly accelerated; and the breeding method has importance values on promoting economic development by forming high-quality dendrobium officinale variety and expanding the resources of the dendrobium officinale seeds.

Description

technical field [0001] The invention relates to a method for cultivating cold-resistant dendrobium officinale seedlings in Sanqing Mountains. Background technique [0002] Dendrobium officinale Kimura et Migo is a perennial epiphytic herb of the genus Dendrobium orchidaceae, also known as black knot grass, which is a precious medicinal plant. Dao Zang is known as "the first of the nine Chinese celestial grasses", and the folks call it "life-saving celestial grass". Dry polydipsia, deficiency of stomach yin, lack of food and retching, persistent deficiency and fever after illness, hyperactivity of fire due to deficiency of yin, fatigue and heat due to bone steaming, dark eyesight, weakness of muscles and bones; modern research shows that Dendrobium officinale contains polysaccharides, alkaloids, amino acids and other ingredients can significantly improve the immune function of the body, and have the effects of nourishing the stomach, nourishing the kidneys, nourishing yin an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/02A01H1/04
Inventor 刘慧芬
Owner 江西瀚野生物科技股份有限公司
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