Unlock instant, AI-driven research and patent intelligence for your innovation.

A clamp-type zn(ii) metal-organic complex, its preparation method and its application in visual recognition of adenosine triphosphate

A metal-organic and complex technology, which is applied in the direction of zinc-organic compounds, through chemical reaction of materials, and material analysis through observation of the influence of chemical indicators, can solve problems that are not simple, direct, fast, and effective. Accurate identification of adenosine triphosphate, cumbersome detection steps and other issues

Inactive Publication Date: 2017-02-22
FUDAN UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods require the use of sophisticated large-scale instruments and cumbersome detection steps
In addition, there is no simple, direct, fast and effective method to identify ATP, especially the related reports of naked eye visual recognition

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A clamp-type zn(ii) metal-organic complex, its preparation method and its application in visual recognition of adenosine triphosphate
  • A clamp-type zn(ii) metal-organic complex, its preparation method and its application in visual recognition of adenosine triphosphate
  • A clamp-type zn(ii) metal-organic complex, its preparation method and its application in visual recognition of adenosine triphosphate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Preparation of pincer Zn (II) metal organic complex 1:

[0022] In a 250 mL round bottom flask, add terpyridine S1 (2.993 g, 10 mmol), zinc nitrate hexahydrate (3.57 g, 12 mmol), and anhydrous methanol, respectively. The reaction was stirred at room temperature for 24 hours. A white solid formed in the reaction system. The white solid was obtained by filtration, washed three times with anhydrous methanol, and dried in vacuo to obtain 3.91 g of the corresponding pincer Zn(II) metal organic complex 1. The yield was 80%.

[0023] High resolution mass spectrometry (HR-MS, ESI): m / z 425.0228 (calcd, [M-NO 3 ] + ); 425.0223(found, [M-NO 3 ] + ).

Embodiment 2

[0024] Example 2: Using pincer-type Zn (II) metal organic complex 1 and 0.25 times the molar equivalent of adenosine triphosphate mixed in water, the conventional operation of the gel preparation after heating and cooling, the formation of two-component metal hydrogel formation to achieve the Naked eye visual recognition of adenosine triphosphate:

[0025] In a 5 mL white sample bottle, add 20 mg pincer-type Zn(II) metal-organic complex 1 and 1 mL water successively, seal it with a polyvinyl fluoride cap, and heat the sample bottle with a heat gun. When the solution inside becomes transparent, that is, the clamp-type Zn(II) metal-organic complex 1 is completely dissolved, the heating is stopped, and the sample bottle is left to stand for a while. It was observed that a white precipitate was formed in the sample bottle, and no metal hydrogel was formed. Add adenosine triphosphate in a molar equivalent of 0.25 times that of the pincer-type Zn(II) metal-organic complex 1 to the ...

Embodiment 3

[0026] Example 3: Using pincer-type Zn(II) metal organic complex 1 and 0.25 times the molar equivalent of adenosine diphosphate mixed in water, the conventional operation of gel preparation after heating and cooling, the situation of forming a precipitate:

[0027] In a 5 mL white sample bottle, add 20 mg pincer-type Zn(II) metal-organic complex 1 and 1 mL water successively, seal it with a polyvinyl fluoride cap, and heat the sample bottle with a heat gun. When the solution inside becomes transparent, that is, the clamp-type Zn(II) metal-organic complex 1 is completely dissolved, the heating is stopped, and the sample bottle is left to stand for a while. It was observed that a white precipitate was formed in the sample bottle, and no metal hydrogel was formed. Add adenosine diphosphate in a molar equivalent of 0.25 times that of the clamp-type Zn(II) metal-organic complex 1 to the sample bottle again, seal it again with a polyvinyl fluoride cap, and heat the sample bottle wit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of application of visual recognition of small biological molecules, and particularly relates to preparation of a novel pincer-like Zn(II) metal organic complex and application of the pincer-like Zn(II) metal organic complex to naked-eye visual recognition of triphosadenine. The expression of the pincer-like Zn(II) metal organic complex is NNN-Zn-(NO3)2, wherein NNN represents a terpyridyl ligand coordinated with Zn. Under a simple operation (heating / cooling) condition, when the pincer-like Zn(II) metal organic complex 1 is mixed with 0.25 molar equivalent of triphosadenine in water, a kind of two-component metal hydrogel can be formed through the conventional get preparation operations of heating and cooling. While when the pincer-like Zn(II) metal organic complex 1 is mixed with 0.25 molar equivalent of adenosine diphosphate and adenosine monophosphate in water, no metal hydrogel but sediments can be generated through the conventional gel preparation operations such as heating and cooling. The invention develops a fast, simple and convenient new method for naked-eye visual recognition of triphosadenine for the first time.

Description

technical field [0001] The invention belongs to the technical field of visual recognition of biological small molecules, and specifically relates to the preparation of a novel pincer-type Zn (II) metal-organic complex and its realization of adenosine triphosphate naked eye based on the selective formation of a two-component gel. method of visual recognition. When the pincer-type Zn(II) metal-organic complex is mixed with adenosine triphosphate in water, a stable two-component metal hydrogel can be easily prepared by the conventional operation of gel preparation with heating and cooling; and adenosine diphosphate (ADP) And adenosine monophosphate (AMP) could not form a stable gel with Zn compound, therefore, a naked-eye visual identification method of adenosine triphosphate was developed. Background technique [0002] Adenosine triphosphate, namely adenosine triphosphate (ATP), is a high-energy phosphate compound, which realizes the process of energy storage and energy relea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07F3/06G01N21/82
Inventor 涂涛刘丛房微魏陆正未
Owner FUDAN UNIV