Clonorchis sinensis real-time fluorescence polymerase chain reaction (PCR) detection kit and detection method

Abstract

The invention provides a clonorchis sinensis real-time fluorescence polymerase chain reaction (PCR) detection kit which comprises a PCR specific primer and a fluorescent probe, wherein the PCR specific primer consists of a clonorchis sinensis ribosome 18S upstream primer having a base sequence shown as SEQ. ID. No. 1 in a sequence list and a clonorchis sinensis ribosome 18S downstream primer having a base sequence shown as SEQ. ID. No. 2 in the sequence list; the fluorescent probe has a base sequence shown as SEQ. ID. No. 3 in the sequence list; and the 5' end of the fluorescent probe is labeled with a fluorescent reporter group and the 3' end is labeled with a fluorescent quenching group. Compared with an existing detection method, the clonorchis sinensis real-time fluorescence PCR detection method disclosed by the invention can be used for reflecting the process of target gene amplification in real time and directly showing the copy number of the target group according to internal parameters or external parameters; the detection method has the advantages of being high in sensitivity, strong in specificity, short in cycle, high in flux and the like; meanwhile, the complex operation of gel electrophoresis is avoided by detecting a fluorescence signal by virtue of an optical system; and the detection method can be used for reducing the occurrence rate of false positive and potential hazard to human bodies and environment, and the detection method can be also used for shortening detection time.

Description

technical field [0001] The invention belongs to the technical field of molecular biology detection, and in particular relates to a clonorchis sinensis real-time fluorescent PCR detection kit and a detection method. Background technique [0002] According to the monitoring results of foodborne parasitic diseases in parasite clinics in Shenzhen and the monitoring results of foodborne parasite projects in the food pollutant monitoring network in Shenzhen in recent years, the infection rate of clonorchis sinensis in aquatic products is gradually increasing. Therefore, it is necessary for us to detect food-borne parasites such as Clonorchis sinensis in aquatic products circulating on the market. The existing traditional detection methods mainly use morphological identification techniques, which are cumbersome, time-consuming, and low in detection rate. Contents of the invention [0003] The purpose of the embodiments of the present invention is to overcome the above-mentioned ...

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Problems solved by technology

Existing traditional detection methods mainly use morphological identification technology, which is cumbersome, time-consuming, and low detection rate

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