Stochastic optical reconstruction microscopy system and method based on LED light sources
An LED light source, random optics technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of high cost of laser light source, limited wide application, difficult to reduce cost, etc., to increase system cost, ensure imaging quality, and facilitate light strong effect
Inactive Publication Date: 2015-04-29
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Moreover, many foreign manufacturers have launched mature STORM products, such as N-STORM of Nikon, STORM of Zeiss, etc., and the prices range from RMB 1.8 to 3 million according to different configurations. The price limits the wide application
[0006] At present, all domestic and foreign studies on stochastic optical reconstruction microscopy, as well as the above-mentioned mature commercial equipment, use laser light sources for the construction of experimental devices. Although high resolution, signal-to-noise ratio and imaging speed are obtained, the cost of laser light sources is High, and a system requires multiple laser light sources, it is difficult to reduce the cost
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[0036] Preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings.
[0037] In order to achieve the purpose of the present invention, as Figure 4 and 6As shown, in some embodiments of the random optical reconstruction microscopic imaging system based on LED light source of the present invention, an object stage (i.e. object plane OBJ), CCD detector and objective lens (i.e. dichroic mirror DM and Optical filter F), as an improvement, it is also provided with four LED light sources 1-4, which respectively form four light transmission paths, and a filter is also arranged between the LED light source 2 and the dichroic mirror DM at the objective lens Sheet F, dichroic mirror DM and multiple lenses L, the light of LED light source 2 passes through the filter F, dichroic mirror DM and multiple lenses L to the dichroic mirror DM at the objective lens, the four LEDs The light sources 2 are also connected to the con...
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The invention discloses a stochastic optical reconstruction microscopy system based on LED light sources. The system comprises an objective table, a CCD (charge coupled device) detector and an objective lens, and further comprises the plurality of LED light sources and a control circuit, wherein the LED light sources form a plurality of light transmission paths respectively; light filters, dichroic mirrors and a plurality of lenses are arranged between the LED light sources and the objective lens; light of the LED light sources passes through the light filters, the dichroic mirrors and the lenses to reach the objective lens; the control circuit is connected with the LED light sources and used for adjusting the light intensity of the LED light sources and switching the LED light sources. Meanwhile, the invention further discloses a stochastic optical reconstruction microscopy method based on the LED light sources. Compared with the prior art, the cost can be greatly reduced while the imaging quality is guaranteed.
Description
technical field [0001] The invention relates to a stochastic optical reconstruction microscopic imaging technology in the field of microscopic technology, in particular to a stochastic optical reconstruction microscopic imaging system and method based on an LED light source. Background technique [0002] Stochastic Optical Reconstruction Microscopy (hereinafter referred to as STORM) is an important super-resolution microscopy method. The STORM process includes several cycles, and only a small amount of random fluorescent molecules emit fluorescence in each cycle. The method of function fitting realizes single-molecule fluorescence imaging (FIONA), and the images are combined after several cycles to realize super-resolution imaging. The lateral resolution of STORM achieved by Michael J Rust et al. can reach 20nm. [0003] Specifically, a Cy3-Cy5 fluorescent molecule pair is used as an example for illustration, in which Cy3 serves as an excitation substance and Cy5 serves as ...
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Inventor 杨西斌熊大曦李辉
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI