Three-dimensional code biological detection chip based on surface-enhanced Raman scattering (SERS) microflow platform as well as preparation method and detection method of biological detection chip

A biological detection and three-dimensional code technology, applied in the fields of spectroscopy and biological analysis, can solve the problems of complex structure, insufficient information, complicated operation, etc., and achieve the effects of large information capacity, simple preparation and detection methods, and wide application range.

Active Publication Date: 2015-04-29
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the widely used high-throughput biological detection system of two-dimensional code has the disadvantages of complex structure, cumbersome operation, and insufficient information.

Method used

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  • Three-dimensional code biological detection chip based on surface-enhanced Raman scattering (SERS) microflow platform as well as preparation method and detection method of biological detection chip
  • Three-dimensional code biological detection chip based on surface-enhanced Raman scattering (SERS) microflow platform as well as preparation method and detection method of biological detection chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: SERS probes were prepared on the basis of gold-core silver-shell nanorods, and high-throughput protein detection was realized by utilizing the specific reaction between antibodies and antigens.

[0030] 1) Preparation of SERS probes.

[0031] To prepare the gold seeds first, mix 2.5 ml of 0.2 M cetyltrimethylammonium bromide (CTAB) solution with 1.5 ml of 1.0 mM tetrachloroauric acid solution at room temperature, stir vigorously and add 0.6 ml of ice-cold 0.01 M hydroboration Sodium solution, stop stirring after 2 minutes to obtain a brown-yellow seed solution. Then prepare a growth solution, add the following reagents in sequence to 50ml 0.2M CTAB solution at room temperature: 2-4ml 4mM silver nitrate solution, 5ml 15mM tetrachloroauric acid solution, 45ml deionized water, and stir slowly evenly. Then 1.5ml-3ml of 0.08M ascorbic acid was added until the solution became colorless. Finally, 1 ml of seed solution was added, and the gold nanorod solution was o...

Embodiment 2

[0040] Example 2: SERS probes were prepared on the basis of gold-core silver-shell nanorods, and high-throughput drug detection was realized by utilizing the specific reaction between antibodies and antigens. :

[0041] 1) Preparation of SERS probes.

[0042] To prepare the gold seeds first, mix 2.5 ml of 0.2 M cetyltrimethylammonium bromide (CTAB) solution with 1.5 ml of 1.0 mM tetrachloroauric acid solution at room temperature, stir vigorously and add 0.6 ml of ice-cold 0.01 M hydroboration Sodium solution, stop stirring after 2 minutes to obtain a brown-yellow seed solution. Then prepare a growth solution, add the following reagents in sequence to 50ml 0.2M CTAB solution at room temperature: 2-4ml 4mM silver nitrate solution, 5ml 15mM tetrachloroauric acid solution, 45ml deionized water, and stir slowly evenly. Then 1.5ml-3ml of 0.08M ascorbic acid was added until the solution became colorless. Finally, 1 ml of seed solution was added, and the gold nanorod solution was o...

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Abstract

The invention discloses a three-dimensional code biological detection chip based on a surface-enhanced Raman scattering (SERS) microflow platform as well as a preparation method and a detection method of the biological detection chip. The biological detection chip comprises a glass substrate, biological recognition molecules and microfluidic channels which are arranged from bottom to top, wherein the biological recognition molecules are distributed on the glass substrate in a strip-shaped form; in a direction perpendicular to the strip-shaped biological recognition molecules, the multiple microfluidic channels are stuck and distributed in parallel manner; and to-be-detected samples and SERS probes are filled into the microfluidic channels sequentially. By collecting SERS spectrums in various detection regions, the to-be-detected samples can be quantitatively analyzed, so as to obtain the contents of various components. By virtue of the biological detection chip disclosed by the invention, the rapid, high-sensitivity and automatic quantitative analysis and detection can be achieved on various components in a great amount of the to-be-detected samples.

Description

technical field [0001] The invention relates to the fields of spectroscopy and biological analysis, in particular to the design and application of a three-dimensional code high-throughput biological detection chip based on surface-enhanced Raman scattering and microfluidics. Background technique [0002] With the rapid development of genomics and proteomics, the fields of biomedical testing, new drug development, and environmental monitoring have put forward multiple and high-throughput technical requirements for the detection of a large number of biomolecules. How to realize the convenient, rapid and highly sensitive analysis and detection of different components in a large number of samples is an urgent problem to be solved. [0003] The microfluidic chip (lab-on-a-chip) flexibly combines and integrates various unit modules on an overall controllable micro platform, which can greatly shorten the sample processing time, significantly improve the detection sensitivity, and r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 王著元崔一平伍磊范可泉宗慎飞
Owner SOUTHEAST UNIV
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