A method for rapidly propagating its seedlings by using the hypocotyls of Zouma embryo

A technology of embryo and hypocotyl, applied in the field of plant biology, can solve the problems that have not been reported in the research, and achieve the effect of broad application prospect, strong practical operability and large yield

Inactive Publication Date: 2016-09-28
罗定市百草源种养专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the research on Zoumatai focuses on the fields of biology, ecological resources and effective component analysis, but the research on the tissue culture technology of Zoumatu seedlings has not been reported yet.

Method used

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  • A method for rapidly propagating its seedlings by using the hypocotyls of Zouma embryo
  • A method for rapidly propagating its seedlings by using the hypocotyls of Zouma embryo
  • A method for rapidly propagating its seedlings by using the hypocotyls of Zouma embryo

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Effect test

preparation example Construction

[0045] Preparation of culture medium: Prepare the culture medium according to conventional methods, adjust the pH value to 5.8, sterilize at 124°C for 25 minutes after aliquoting, and set aside.

[0046] Seed collection, processing and disinfection: The mature fruits with deep red peel and no damage from diseases and insect pests were collected from Zoumatai Germplasm Garden of Guangxi Institute of Botany. The pulp and seed coat were removed, cleaned with tap water, and full-grown seeds were selected. On the ultra-clean workbench, first soak it in an alcohol solution with a volume fraction of 70-75% for 30 seconds, then soak it in a sodium hypochlorite solution with a mass fraction of 15% for 10-15 minutes, and then rinse it with sterile water 5 times. Blot the moisture on the surface with sterile filter paper for later use.

Embodiment 1

[0048] Utilize the method for rapidly propagating its seedling of Zouma embryo hypocotyl, comprises the following specific steps:

[0049] (1) Select well-grown Zoumatai seeds, add gibberellin aqueous solution with a concentration of 120 mg / L after surface disinfection and sterilization, the volume ratio of the solution to the seeds is 5:1, at a temperature of 35±2°C and a rotational speed of Soak for 24 hours on a shaker at 130~138 rpm, and rinse with sterile water.

[0050] (2) Inoculate the treated seeds in the germination medium, the germination medium is: MS+6-BA 0.5 mg / L+NAA 0.1 mg / L, at the same time add agar powder 5 g / L, sucrose 30 g / L , 0.2% activated carbon, pH 5.8; culture conditions are dark culture at a temperature of 28±2°C. After 5-7 days of culture, the radicle germinates and elongates, and the hypocotyl elongates 2-3cm after 40-50 days, and the cotyledons unfold to form young shoots. The germination rate reaches 97.5%, see figure 1 .

[0051] (3) After th...

Embodiment 2

[0056] Utilize the method for rapidly propagating its seedling of Zouma embryo hypocotyl, comprises the following specific steps:

[0057] (1) Select well-grown Zoumatai seeds, add gibberellin aqueous solution with a concentration of 150 mg / L after surface disinfection and sterilization, the volume ratio of the solution to the seeds is 5:1, at a temperature of 35±2°C and a rotational speed of Soak for 24 hours on a shaker at 130~138 rpm, and rinse with sterile water.

[0058] (2) Inoculate the treated seeds in the germination medium, the germination medium is: MS+6-BA 1.0 mg / L+NAA 0.2 mg / L, at the same time add agar powder 5 g / L, sucrose 30 g / L , 0.2% activated carbon, pH 5.8; culture conditions are dark culture at a temperature of 28±2°C. The germination rate was 85.2% after 40 days of cultivation.

[0059] (3) After the seeds germinate, cut the immature hypocotyls and insert them into the adventitious bud induction medium to induce adventitious buds. The hypocotyl adventit...

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Abstract

The invention discloses a method for rapidly propagating seedlings by using the hypocotyls of the young embryos of the Zouma fetus, which comprises the following steps: selecting the fully grown Zouma fetus seeds, sterilizing the surface, treating them with gibberellin, and inoculating them on the germination medium Induce the formation of young buds, cut the hypocotyls of the young buds and insert them into the adventitious bud induction medium to form adventitious buds, then cut the adventitious buds into small sections with 1 to 2 axillary buds and put them into the bud proliferation medium for proliferation and culture, and then cut the adventitious buds to grow vigorously 1. A single bud with a length of 3 to 4 cm is inserted into the rooting medium to induce rooting, and the seedlings are hardened and transplanted. The invention has the advantages that a small amount of Zouma tire material can be rapidly multiplied to produce a large number of adventitious buds, and the survival rate is as high as 82.5%, which provides an effective way for the industrialized seedling cultivation of Zouma tire.

Description

technical field [0001] The invention belongs to the field of plant biology technology, and in particular relates to a method for rapidly propagating seedlings by using the hypocotyls of the young embryos of Zouma fetus. Background technique [0002] Goofy ( Ardisia gigantifoliaStapf ) is a commonly used bruises medicine in the folk. The rhizome and the whole plant have the functions of dispelling wind and strengthening bones, promoting blood circulation and removing stasis, reducing swelling and pain, hemostasis and granulation. It can treat rheumatoid arthritis, muscle and bone pain, bruises, postpartum congestion , hemiplegia, carbuncle ulcer, etc. Modern studies have proved that Zoumatai is rich in triterpenoid saponins, petracenin derivatives, volatile oils and other compounds, among which triterpenoid saponins have a strong inhibitory effect on the activity of various tumor cells. For example: the oleanane-type pentacyclic triterpene saponin compound AG4, which is ext...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01C1/00A01C1/08
Inventor 唐凤鸾黄宁珍何金祥赵健毛世忠
Owner 罗定市百草源种养专业合作社
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