Human organ freezing protective solution

A technology of cryoprotective liquid and human organs, which is applied in the field of medical research, can solve the problems of inability to adapt to the freezing technology of human organs and large cell damage, and achieve the effects of low preparation cost, easy availability of raw materials, and reduced damage

Inactive Publication Date: 2015-05-06
陈德琼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to overcome the fact that the existing domestic refrigerants cannot adapt to the research requirements of human organ freezing technology, and the cell damage is too large in the vitrification method, the present invention hopes to provide a new type of cryoprotective liquid for human organs, which can greatly reduce the number of cells in the freezing process. damage to

Method used

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Embodiment Construction

[0008] specific implementation plan

[0009] Formula: 15% dimethyl sulfoxide, 15% ethylene glycol, 10% glycerol, 14% sucrose, 10% polyethylene glycol, 11% polysucrose, and the balance is conventional solvents. Dimethyl sulfoxide Dissolve ethylene glycol in sterilized distilled water, add other remaining materials and mix evenly, add deionized water, adjust the pH value to 7.4 with one equivalent of sodium hydroxide, heat to cause excess water to evaporate, and obtain the human organ of the present invention Cryoprotectant solution, bottled, steam sterilized.

[0010] Described conventional solvent is deionized water, hydrochloric acid, sodium hydroxide.

[0011] In a word, the present invention has good effect, and also has low cost, simple production procedure, good stability, and can be popularized and used.

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Abstract

In order to enable human organ tissue cells to be not damaged by ice crystals generated at a low temperature in a temperature dropping process to the greatest extent, a freezing protective solution is required to add in a human organ cooling process, so that cell activities are improved during freezing; however, more than 45% of the cells are damaged by an existing freezing protective solution in the cooling process. The human organ freezing protective solution comprises active ingredient of the following raw materials in percentage by weight: 12-18% of dimethyl sulfoxide, 14-20% of ethylene glycol, 8-12% of glycerol, 12-15% of sucrose, 8-12% of polyethylene glycol and 9-15% of polysucrose. The human organ freezing protective solution can be used for greatly reducing damages, in a freezing process, of organ tissue cells during a process of implementing a vitrification freezing method.

Description

technical field [0001] The invention belongs to a cryoprotective liquid for human organs, which is suitable for the field of medical research. Background technique [0002] At present, vitrification technology, as a method of rapidly freezing cells or tissues, has attracted widespread attention with the development of assisted reproductive technology. Vitrification technology has been extended and applied to the research of human organ freezing abroad. This technology has the advantages of fast freezing speed, small freeze-thaw damage, simple operation, etc., and can improve the survival rate after freezing and thawing. In practice, freezing human organs is extremely difficult and faces many technical obstacles. When the low temperature reaches a certain low temperature, the vitality of human tissue cells can be preserved, but the cells often die during the cooling process. Because the cells are rich in water, ice crystals will form during the cooling process and cause dam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 陈德琼
Owner 陈德琼
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