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Application of long-chain non-coding RNA molecule SNHG18 in preparing medicine for treating brain glioma

A long-chain non-coding, brain glioma technology, which is applied in the application field of long-chain non-coding RNA molecule SNHG18 in the preparation of drugs for the treatment of brain glioma, and can solve the problems that no further research reports on functional and structural characteristics have been found.

Inactive Publication Date: 2015-05-06
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there is no further research report on its function and structural characteristics

Method used

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  • Application of long-chain non-coding RNA molecule SNHG18 in preparing medicine for treating brain glioma
  • Application of long-chain non-coding RNA molecule SNHG18 in preparing medicine for treating brain glioma
  • Application of long-chain non-coding RNA molecule SNHG18 in preparing medicine for treating brain glioma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: SNHG18 is highly expressed in radiation resistant glioma cell lines.

[0033] Experimental materials: glioma cell lines M059K and MO59J, purchased from ATCC cell bank. U87 and U118 cell lines were purchased from the cell bank of Shanghai Institute of Cell Research, Chinese Academy of Sciences.

[0034] Experimental method and result analysis:

[0035] 1. Cell culture: The cells are resuscitated in a 37°C water bath, and then inoculated into a cell culture flask. M059K and MO59J cells are cultured using DMEM-F12 medium (FBS, Gibco, Australia) containing 10% fetal bovine serum, and U87 and U118 cells are cultured using DMEM medium (FBS, Gibco, Australia) containing 10% fetal bovine serum. The incubator condition is 37℃, 5% CO 2 .

[0036] 2. Gene chip: Arraystar Human LncRNA Microarray V3.0 chip is used to analyze the LncRNA expression profile of glioma cell lines M059J (sensitive strain) and M059K (resistant strain) derived from the same parent and with different ra...

Embodiment 2

[0046] Example 2: SNHG18 promotes the proliferation of nasopharyngeal carcinoma cells.

[0047] 1. Cell transfection: the glioma cells were pressed 3×10 the day before the transfection experiment 5 The density is inoculated in a 6-well plate, and about 2ml of antibiotic-free medium is added to each well, so that the cell density can reach about 70-80% during transfection. Take 5μl / well of Lipofectamin 2000 (Invitrogen, Carlsbad, California, USA), dilute with 250μl Opti-MEM (Opti-MEMI Reduced Serum Medium), mix gently and incubate at room temperature for 5min. Take 8μl SNHG18 / siRNA or Vector / NC (Jikai, Shanghai), dilute with 250μl Opti-MEM (Opti-MEMI Reduced Serum Medium), mix gently and incubate at room temperature for 5min. The diluted Lipofectamin2000 is gently mixed with the diluted SNHG18 / siRNA or Vector / NC, and the mixture is allowed to stand at room temperature for 20 minutes to form a transfection reagent mixture. Add the mixture to the wells containing cells and opti-MEM...

Embodiment 3

[0050] Example 3: The effect of SNHG18 on the apoptosis of glioma cells

[0051] Flow cytometric detection of apoptosis: spread the cells evenly in a 6-well plate. After 24 hours, the SNHG18 NC / siRNA is incubated with lipo2000 and opti-MEMI (about 1500μl) and then transferred into the cells. After 6 hours of transfection, the cell culture medium Replace with fresh culture medium. After 48 hours of transfection, the collected cells were stained with annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit (Sigma-Aldrich, USA). Flow cytometry was used to detect the apoptosis of H1299 cells.

[0052] The results showed that the apoptosis rate of glioma cells that interfered with the expression of SNHG18 was significantly higher than that of the control group. Statistical analysis was performed using SPSS13.0 software, and the two sample means were compared using two independent-sample t test (Independent-Sample T Test) and one-sample t test. P values ​​ Figure 4A-4B ).

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Abstract

The invention discloses an application of a long-chain non-coding RNA molecule SNHG18 in preparing a medicine for treating brain glioma, and in particular discloses an application in preparing a medicine for enhancing the radiosensitivity of a brain glioma cell. The application disclosed by the invention, from the new view of 1ncRNA, offers a new basis and a new idea for illuminating the malignant proliferation of malignant glioma and the molecular mechanism of radio-resistance, and provides a certain application value for the aspect of drug target. Therefore, the SNHG18 can be used for preparing a medicine for treating brain glioma, in particular for preparing a medicine for enhancing the radiosensitivity of a brain glioma cell.

Description

Technical field [0001] The present invention relates to a new use of the long-chain non-coding RNA molecule SNHG18, in particular to the application of SNHG18 in the preparation of drugs for treating glioma. Background technique [0002] Glioma is the most common intracranial tumor in humans. Malignant gliomas are treated with comprehensive therapies, including surgical resection, radiation therapy, and chemotherapy. A large number of clinical studies have shown that postoperative radiotherapy for malignant glioma can significantly improve the survival rate of patients, and radiotherapy has an important position in the treatment of malignant glioma. However, malignant gliomas after comprehensive treatment are still prone to relapse, and the curative effect is unsatisfactory. Further exploration of new mechanisms and new ways to improve the treatment of malignant glioma is an important content of current malignant glioma research. [0003] Tumor radiotherapy is a complex biologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P35/00
Inventor 袁亚维王玮刘英杜莎莎杨开军谢国柱
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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