Synergistic elution remediation method for soil contaminated by high-abundance resistance genes combined with tetracycline and heavy metals
A technology of resistance gene and repair method, applied in the restoration of contaminated soil, etc., can solve the problems of less attention to soil, poor repair effect, poor environmental friendliness of chemical eluents, etc., and achieve broad application prospects and broad spectrum High reliability and low cost effect
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Embodiment 1
[0032] For total tetracycline concentrations greater than 1 mg kg -1 and less than 10 mg kg -1 , Cu concentration greater than 50 mg kg -1 And less than 100 mg kg -1 , Zn concentration greater than 50 mg kg -1 And less than 100 mg kg -1 , the abundance of tetracycline resistance gene is greater than 1×10 5 less than 1×10 6 Copy number of composite contaminated soil:
[0033] The test soil was collected from a site polluted by the accumulation of feces from a pig farm in Taizhou City, Jiangsu Province. The soil samples were air-dried, the impurities were sorted out, and the soil samples were passed through a 10-mesh sieve for later use. The basic physical and chemical properties of the soil are pH 6.6, organic matter content 47.3 g kg -1 , total nitrogen 3.6g kg -1 , available nitrogen 89.5 mg kg -1 , total phosphorus 0.9 g kg -1 , available phosphorus 85.4 mg kg -1 . The mechanical composition of the soil is 5.3 wt.% sand, 61.8 wt.% loam, and 32.9 wt.% clay. The ...
Embodiment 2
[0036] For total tetracycline concentrations greater than 10 mg kg -1 and less than 50 mg kg -1 , Cu concentration greater than 100 mg kg -1 And less than 200 mg kg -1 , Zn concentration greater than 100 mg kg -1 And less than 200 mg kg -1 , the abundance of tetracycline resistance gene is greater than 1×10 6 less than 1×10 7 Copy number of composite contaminated soil:
[0037] The test soil was collected from the polluted soil of a broiler farm in Bengbu City, Anhui Province. The soil samples were air-dried, the impurities were sorted out, and the soil samples were passed through a 10-mesh sieve for later use. The basic physical and chemical properties of the soil are pH 6.9, organic matter content 26.6 g kg -1 , total nitrogen 3.1 g kg -1 , available nitrogen 58.7 mg kg -1 , total phosphorus 0.9 g kg -1 , available phosphorus 61.3 mg kg -1 . The mechanical composition of the soil is 4.2 wt.% sand, 53.6 wt.% loam, and 42.2 wt.% clay. The background contamination...
Embodiment 3
[0040] For total tetracycline concentrations greater than 50 mg kg -1 , Cu concentration greater than 200 mg kg -1 , Zn concentration greater than 200mg kg -1 , the abundance of tetracycline resistance gene is greater than 1×10 7 Copy number of composite contaminated soil:
[0041] The test soil was collected from the polluted soil of a poultry farm in Ningbo City, Zhejiang Province. The soil samples were air-dried, the impurities were sorted out, and the soil samples were passed through a 10-mesh sieve for later use. The basic physical and chemical properties of the soil are pH 6.1, organic matter content 15.7 g kg -1 , total nitrogen 1.2 g kg -1 , available nitrogen 34.1 mg kg -1 , total phosphorus 0.3 g kg -1 , available phosphorus 42.4 mg kg -1 . The mechanical composition of the soil is 7.8 wt.% sand, 58.1 wt.% soil, and 34.1 wt.% clay. The background contamination concentrations of total tetracycline, Cu, Zn and tetracycline resistance gene in soil were 61.44 ±...
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Application Information
- IPC
- B09C1/02
- Inventors
- 叶茂; 谢珊妮