Method for guiding expression of major capsid proteins L1 of HPV16 (human papilloma virus 16) in pichia pastoris by using formaldehyde dehydrogenase promoter

Abstract

The invention relates to a method for guiding the expression of major capsid proteins L1 of HPV16 (human papilloma virus 16) in pichia pastoris by using a formaldehyde dehydrogenase promoter, wherein the expression of major capsid proteins L1 of HPV16 is controlled by a pichia pastoris formaldehyde dehydrogenase promoter. The method disclosed by the invention mainly comprises the following steps: i) building a pichia pastoris expression vector guided by a pichia pastoris formaldehyde dehydrogenase promoter; and ii) under the guidance of the formaldehyde dehydrogenase promoter, expressing the major capsid proteins L1 of HPV16 in different carbon / nitrogen source combined culture media. According to the invention, based on a pichia pastoris expression system, and starting with the regulating characteristics of the formaldehyde dehydrogenase promoter, the optimized expression of major capsid proteins L1 of HPV16 is implemented in the different carbon / nitrogen source combined culture media according to the characteristics of the promoter, so that the efficient expression of the major capsid proteins L1 of HPV16 is realized, thereby obtaining a low-cost virus-like particle preparation technology and implementing the low-cost preparation of human papilloma virus vaccines.

Description

Technical field [0001] The present invention belongs to the field of biological engineering technology, which involves the use of the use of Bichic yeast formaldehyde dehydrogenase (FLD) in the use of the chinensis host cells in the hyaluronic types of the hypolitic yeast host cells.) Expressive method. Background technique [0002] Bi Chi yeast expression system can effectively secrete heterogeneous proteins and perform post -translation modification. The operation is easy to achieve high -density fermentation, and does not produce internal toxins and mammalian nucleic acid components. Therefore, it is a widely used protein.Expression system.Transcription is one of the most critical links of gene expression regulation. The promoter can significantly affect the beginning, level, and persistence of heterogeneous transcription, thereby directly affecting the expression efficiency of heterogeneous protein.The most commonly used and classic promoter in Bi Chi yeast is type of mosquit...

AI Technical Summary

Problems solved by technology

[0004] Although the constitutive promoter simplifies the production steps, if a higher protein yield is obtained, the growth of the bacteria is required to be higher, and its regulatory pathway is relatively simple, and the types of protein expression are limited.

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