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Dyeing method of fish bones

A dyeing method, bone dyeing technology, applied in the field of fish, can solve the problems of lack of convincing experimental results, inconspicuous red coloring, loss of repeatability, etc., and achieve the effects of improving manual work efficiency, easy access, and reducing scientific research costs

Inactive Publication Date: 2015-05-06
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the course of the experiment, it may be due to improper operation (conditional control of time, temperature, etc.) that the staining will be abnormal, such as hard bone stained blue, red staining is not obvious, etc.
The emergence of this kind of problem will cause unpredictable errors in the research
At the same time, due to the use of a method on different batches, the results are repeated and the repeatability is lost, which makes the experimental results less convincing

Method used

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  • Dyeing method of fish bones
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  • Dyeing method of fish bones

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The dyeing method of the fish skeleton provided by the present embodiment, such as figure 1 , including the following steps:

[0045] S1. Sample pretreatment: Freshly collected fish samples need to be immersed in 10% neutral formalin, and the preservation solution should be replaced after 24 hours, and transferred to 4°C for preservation;

[0046] Condition: 4°C; solution: sample (V / g) = 200mL: 1g,

[0047] Preparation method: 200 mL formalin, 1800 mL deionized water, 8 g NaH 2 PO 4 ·H 2 O, 13 g Na 2 HPO 4 ;

[0048] Note: Before staining, samples need to be placed in 10% neutral formalin for at least one week.

[0049]S2. Dehydration: According to the sample volume, select a beaker of appropriate size, take the sample out of the preservation solution, and perform the dehydration step. The dehydration steps include:

[0050] 1) Dehydration 1: 70% ethanol solution for 24 hours;

[0051] 2) Dehydration 2: 80% ethanol solution for 24 hours;

[0052] 3) Dehydra...

Embodiment 2

[0080] The dyeing method of the fish skeleton provided by the present embodiment comprises the following steps:

[0081] (1) Sample pretreatment, take fish samples, immerse them in neutral formalin solution, freeze them after immersion;

[0082] The neutral formalin solution is composed of formalin, deionized water, NaH 2 PO 4 ·H 2 O and Na 2 HPO 4- Made of formalin, deionized water, NaH 2 PO 4 ·H 2 O and Na 2 HPO 4 The dosage relationship is 200mL: 1800mL: 8g: 13g, wherein the volume percentage of the neutral formalin solution is 10%.

[0083] After immersing for at least 24 hours, replace the neutral formalin solution and store in a freezer at 0°C for at least one week. The dosage relationship between the fish sample and the neutral formalin solution is 1g: 230mL.

[0084] (2) Dehydration: Take out the fish body samples after cryopreservation, and place them in different concentrations of ethanol solutions for gradual dehydration;

[0085] When dehydrating step by...

Embodiment 3

[0097] The dyeing method of the fish skeleton provided by the present embodiment comprises the following steps:

[0098] (1) Sample pretreatment, take fish samples, immerse them in neutral formalin solution, freeze them after immersion;

[0099] The neutral formalin solution is composed of formalin, deionized water, NaH 2 PO 4 ·H 2 O and Na 2 HPO 4- Made of formalin, deionized water, NaH 2 PO 4 ·H 2 O and Na 2 HPO 4 The dosage relationship is 200mL: 1800mL: 8g: 13g, wherein the volume percentage of the neutral formalin solution is 10%.

[0100] After immersion for at least 24 hours, replace the neutral formalin solution and store at 8°C for at least one week. The dosage relationship between the fish body sample and the neutral formalin solution is 1 g: 250 mL.

[0101] (2) Dehydration: Take out the fish body samples after cryopreservation, and place them in different concentrations of ethanol solutions for gradual dehydration;

[0102]When dehydrating step by step i...

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Abstract

The invention discloses a dyeing method of fish bones. The dyeing method comprises the following steps of: (1) sample pretreatment; (2) dewatering; (3) cartilage dyeing; (4) neutralization; (5) bleaching; (6) enzymolysis; (7) hard-bone dyeing; and (8) storage. The dyeing method disclosed by the invention has the advantages that the stability and the repeatability are high; the adopted equipment and materials are simple, low in cost and easily available, only the condition of a sample needs to be controlled, special persons for looking after the process are not needed, time and labor are saved, the scientific-research cost can be greatly reduced, and the working efficiency of workers can be improved.

Description

technical field [0001] The invention belongs to the technical field of fish, and in particular relates to a method for dyeing fish bones. Background technique [0002] Analyzing the skeleton of fish can study the development sequence and time of the skeleton, as well as determine the deformity of the skeleton of the fish and carry out related statistics. In fact, some methods such as Micro CT scanning and X-ray fluoroscopy can realize the analysis of bones by using large-scale instruments, but these methods have the problem of expensive instruments on the one hand, and the resolution cannot meet the analysis requirements on the other hand, so It is rarely used in the study of fish skeletons. [0003] The staining method is a low-cost, easy-to-implement, effective way to analyze bones. The processed samples can effectively observe, analyze, draw and take pictures of fish bones. Generally, the principle of Alcian blue is easy to combine with cartilage to show blue, and aliz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
Inventor 郑攀龙马振华江世贵何丹张楠傅明俊张殿昌杨丽诗黄建华周发林
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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