Tissue culture and rapid propagation method for American lagerstroemia indica pink velour

A technique for rapid propagation of tissue culture and crape myrtle, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of tissue culture buds turning yellow and falling leaves, slow elongation and growth, and poor quality of seedlings, and achieve root system Well-developed and strong, fast elongation and growth, and low production cost

Inactive Publication Date: 2015-05-13
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If referring to the existing Lagerstroemia indica tissue culture medium for tissue culture and rapid propagation of Lagerstroemia indica, the tissue culture buds will turn yellow and drop leaves, the buds will be small and weak, the elongation and growth will

Method used

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  • Tissue culture and rapid propagation method for American lagerstroemia indica pink velour
  • Tissue culture and rapid propagation method for American lagerstroemia indica pink velour
  • Tissue culture and rapid propagation method for American lagerstroemia indica pink velour

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Embodiment 1

[0040] A method for tissue culture rapid propagation of Lagerstroemia indica, comprising the steps of:

[0041](1) Collection of explants: Select an excellent single plant with beautiful plant shape, fast growth speed, and no pests and diseases as the female parent. The age of the excellent tree is more than 15 years. At around 10:00 in the morning when the weather is fine, cut the semi-lignified branches of the trunk , wash with pure water after removing the leaves, and set aside;

[0042] (2) Disinfection of explants and induction of axillary buds: On the ultra-clean workbench, wash the branches with sterile water, then soak them in 70-75% alcohol solution for 20-30 seconds, pour off the alcohol solution and then sterilize Rinse with water for 1-2 times, then add 0.1% mercuric acid solution, soak for 3-10 minutes, shake continuously during this period, pour out the mercuric acid solution and rinse with sterile water for 5-6 times, after rinsing with sterile water, filter pap...

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Abstract

The invention discloses a tissue culture and rapid propagation method for American lagerstroemia indica pink velour and belongs to the technical field of tissue culture of plants. The method carries out in vitro culture for semi-lignified branches of American lagerstroemia indica pink velour and comprises five steps: selection and disinfection of explants, primary culture, multiplication culture, rooting culture and acclimatization and transplant. The method has the advantages of being high in propagation coefficient and short in culture period and is not limited by seasons, and the induction rate is 88 percent. The multiplication coefficient is 5-6, the multiplied buds is fast in elongated growth, and the buds are 3-4cm after being cultured for 25d. The rooting rate is 100 percent, and the average root number is 4.54 pieces/strain. The tissue culture rooting seedlings are healthy and strong, and the survival rate of transplanting is more than 95 percent. In the actual production application, the method can carry out annual large-scale rapid seedling cultivation, produces healthy, strong and uniform American lagerstroemia indica pink velour seedlings, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture and rapid propagation method of Lagerstroemia indica. Background technique [0002] Lagerstroemia crape myrtle is a small deciduous tree or shrub of the genus Lagerstroemia in the family Lythraceae. It is also known as zinnia, itch tree, and houseplant. It has a beautiful tree shape and blooms in hot summer. . Lagerstroemia indica'Pink Velour' is a new and excellent variety of Lagerstroemia indica'Pink Velour' introduced from the United States by the Hunan Academy of Forestry in 2003. Its new leaves and young shoots are wine red and have a good foliage effect; the flowers are deep pink Color, inflorescence length up to 70cm, dense and elegant, with high ornamental value. The flowering period is from early June to the end of October, up to 5 months. It has strong adaptability, drought tolerance, and can withstand low temperatures of -23...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 邓小梅陈怡佳奚如春董运常胡传伟罗伟聪
Owner SOUTH CHINA AGRI UNIV
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