Tissue culture and rapid propagation method for American lagerstroemia indica pink velour

Abstract

The invention discloses a tissue culture and rapid propagation method for American lagerstroemia indica pink velour and belongs to the technical field of tissue culture of plants. The method carries out in vitro culture for semi-lignified branches of American lagerstroemia indica pink velour and comprises five steps: selection and disinfection of explants, primary culture, multiplication culture, rooting culture and acclimatization and transplant. The method has the advantages of being high in propagation coefficient and short in culture period and is not limited by seasons, and the induction rate is 88 percent. The multiplication coefficient is 5-6, the multiplied buds is fast in elongated growth, and the buds are 3-4cm after being cultured for 25d. The rooting rate is 100 percent, and the average root number is 4.54 pieces / strain. The tissue culture rooting seedlings are healthy and strong, and the survival rate of transplanting is more than 95 percent. In the actual production application, the method can carry out annual large-scale rapid seedling cultivation, produces healthy, strong and uniform American lagerstroemia indica pink velour seedlings, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture and rapid propagation method of Lagerstroemia indica. Background technique [0002] Lagerstroemia crape myrtle is a small deciduous tree or shrub of the genus Lagerstroemia in the family Lythraceae. It is also known as zinnia, itch tree, and houseplant. It has a beautiful tree shape and blooms in hot summer. . Lagerstroemia indica'Pink Velour' is a new and excellent variety of Lagerstroemia indica'Pink Velour' introduced from the United States by the Hunan Academy of Forestry in 2003. Its new leaves and young shoots are wine red and have a good foliage effect; the flowers are deep pink Color, inflorescence length up to 70cm, dense and elegant, with high ornamental value. The flowering period is from early June to the end of October, up to 5 months. It has strong adaptability, drought tolerance, and can withstand low temperatures of -23...

AI Technical Summary

Problems solved by technology

If referring to the existing Lagerstroemia indica tissue culture medium for tissue culture and rapid propagation of Lagerstroemia indica, the tissue culture buds will turn yellow and drop leaves, the buds will be small and weak, the elongation and growth will be slow, and finally the phenomenon of withering and yellowing will appear, and the number of effective buds will be small, etc. situation, resulting in small multiplication coefficient, poor seedling quality, slow propagation speed and high production cost

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