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Method of extracting gambogic acid and neogambogic acid from gamboge

A new technology of gambogic acid and gambogic acid, which is applied in the direction of organic chemistry, can solve the problem of waste of active ingredients, and achieve the effects of simple steps, improved production efficiency and high product purity

Active Publication Date: 2015-05-13
内蒙古昶辉生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But above-mentioned two kinds of methods all are only suitable for isolating one active ingredient of gambogic acid or new gambogic acid, cause waste to the remaining active ingredients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Grind 200g of dried gambogic resin (31.28% gambogic acid content, 12.34% new gambogic acid content) and pass through a 40-mesh sieve. Add the extraction solvent anhydrous ethanol at a solid-to-liquid ratio of 1:20. Under the condition of microwave extraction for 2min. Filter out the extract, concentrate under reduced pressure and recover the solvent to obtain extract A; add an appropriate amount of petroleum ether to the extract A for rinsing, then add 95% ethanol to dissolve, filter, and the filtrate enters simulated moving bed chromatography for separation. The adsorbent is AB-8 macroporous adsorption resin, the desorbent is 25% ethanol aqueous solution, the dosage is 3 times the volume of the resin, the washing area is purified water, the resin adsorption regeneration solvent is 95% ethanol, and the flow rate in the adsorption area is 2BV / h. The flow rate in the desorption zone is 3BV / h, the flow rate in the washing zone is 3BV / h, the flow rate in the regeneration zo...

Embodiment 2

[0020] Grind 500g of dried gambogic resin (31.28% gambogic acid content, 12.34% new gambogic acid content) and pass through a 80-mesh sieve. Add the extraction solvent anhydrous ethanol at a solid-to-liquid ratio of 1:15. Under the condition of microwave extraction 2.5min. Filter out the extract, concentrate under reduced pressure and recover the solvent to obtain extract A; add an appropriate amount of petroleum ether to the extract A for rinsing, then add 95% ethanol to dissolve, filter, and the filtrate enters simulated moving bed chromatography for separation. The adsorbent is DM130 macroporous adsorption resin, the desorbent is 30% ethanol aqueous solution, the dosage is 3 times the volume of the resin, the washing area is purified water, the resin adsorption regeneration solvent is 95% ethanol, the flow rate of the adsorption area is 2BV / h, and the desorption area The flow rate is 3BV / h, the flow rate in the washing area is 3BV / h, the flow rate in the regeneration area i...

Embodiment 3

[0022] Grind 600g of dried gambogic resin (31.28% gambogic acid content, 12.34% new gambogic acid content) and pass through a 60-mesh sieve. Add the extraction solvent anhydrous ethanol at a solid-to-liquid ratio of 1:20. Under the condition of microwave extraction for 3min. Filter out the extract, concentrate under reduced pressure and recover the solvent to obtain extract A; add an appropriate amount of petroleum ether to the extract A for rinsing, then add 95% ethanol to dissolve, filter, and the filtrate enters simulated moving bed chromatography for separation. The adsorbent is AB-8 macroporous adsorption resin, the desorbent is 30% ethanol aqueous solution, and its dosage is 3 times the volume of the resin, the washing area is purified water, the resin adsorption regeneration solvent is 95% ethanol, and the flow rate in the adsorption area is 2BV / h. The flow rate in the desorption zone is 3BV / h, the flow rate in the washing zone is 3BV / h, the flow rate in the regeneratio...

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Abstract

The invention aims at providing a method of efficiently extracting and separating gambogic acid and neogambogic acid from gamboge, serving as a traditional Chinese medicine. The method comprises the following steps: crushing the gamboge, carrying out microwave extraction, and concentrating to obtain an extract A; leaching with petroleum ether, dissolving and filtering with ethanol, and carrying out chromatographic separation by virtue of a simulated moving bed to obtain a component B rich in the gambogic acid and a component C containing the gambogic acid and the neogambogic acid; and recovering a solvent, drying to obtain products, including the gambogic acid with the purity to be above 95 percent and a mixture of 10-20 percent of the gambogic acid and 30-50 percent of the neogambogic acid, in two specifications. The method of extracting the effective components of the gamboge, serving as the traditional Chinese medicine, is simple in step and high in product purity and is capable of simultaneously obtaining the products, including the gambogic acid and the neogambogic acid, in two specifications, fully extracting and separating the effective components from the raw materials and improving the production efficiency while saving the raw material resources.

Description

technical field [0001] The invention relates to a method for extracting gambogic acid and neogambogic acid in the traditional Chinese medicine gambogic. Background technique [0002] Garcinia hanburyi Hook f. is a gelatinous resin that flows out after the trunk of Garcinia hanburyi Hook f. is cut. After drying, it is a yellow-brown columnar or irregular block. It is brittle and brittle. It is native to Southeast Asia and my country. Introduced and cultivated in some areas such as Yunnan, Guangxi, Guangdong and other places. Gambogic contains various components such as Gambogic acid, Neomogenic acid, and Allogambogic acid. In the clinical practice of traditional Chinese medicine, gamboge is mainly used to treat carbuncle, ulcer, eczema, tumor, stubborn ringworm and other diseases. Modern pharmacological studies have shown that gambogic acid has a strong inhibitory effect on a variety of tumor cells, and has less toxic and side effects within the effective dose range. Immune...

Claims

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Application Information

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IPC IPC(8): C07D493/20
CPCC07D493/20
Inventor 梁玉强白易高瑞赵永强张成亮
Owner 内蒙古昶辉生物科技股份有限公司
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