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Material for dual ion charge biased blood cell screening and method for removing white blood cells from a blood sample

A dual-ion, white blood cell technology, applied in separation methods, chemical instruments and methods, filtration separation, etc., can solve problems such as increased bradykinin concentration and allergic reactions

Active Publication Date: 2016-08-31
CHUNG YUAN CHRISTIAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

U.S. Patent No. 5,407,581 discloses a negatively charged filter material for treating blood, using PET (polyethylene terephthalate) fiber material as a substrate, and then grafting a charged polymer on its surface to make the filter The surface of the material is slightly negatively charged, because the positively charged surface is likely to increase the concentration of bradykinin, which is likely to cause allergic reactions during blood transfusion

Method used

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  • Material for dual ion charge biased blood cell screening and method for removing white blood cells from a blood sample
  • Material for dual ion charge biased blood cell screening and method for removing white blood cells from a blood sample
  • Material for dual ion charge biased blood cell screening and method for removing white blood cells from a blood sample

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no. 1 example

[0115] According to the first embodiment of the present invention, a material for screening blood cells with a dual ion charge deviation type is disclosed, which is a copolymer composed of a dual ion structural unit and a charged structural unit, wherein the dual ion structural unit includes at least one positively charged group group and a negatively charged group, the distance between the positively charged group and the negatively charged group is 1 to 5 carbon-carbon bond lengths, and the charged structural unit and the diionic structural unit are randomly arranged to form a double Ion charge bias.

[0116] In the dual ion charge bias type blood cell screening material of the present invention, in addition to the generally known anti-biomolecular adhesion effect, the above-mentioned dual ion structural unit has the ability to provide dual ion by adding positive and negative charged groups The characteristics of ion charge deviation, because the neutral surface includes the...

Embodiment 1

[0209] The material for synthesizing double-ion charge deviation type blood cell screening is a copolymer I composed of a positively charged structural unit (TMA) and a negatively charged structural unit (SA): SAmTMAn (wherein m and n represent the molar ratios of SA and TMA, for example SA8TMA2 means that the molar ratio of SA and TMA is 8:2, and the same notation will be used below).

[0210] Mix TMA ([2-(Methacryloyloxy)ethyl]trimethylammoniumchloride solution) and SA (3-Sulfopropyl methacrylate potassium salt) according to different ratios and stir evenly, and then make the raw material of the monomer unit (that is, the mixture of TMA and SA) 90wt %, add cross-linking agent NMBA (N, N-Methylenebisacryl amide, 96%, manufactured by ACROS Co.) (8wt%) and mix until uniform, then add starter APS (1wt%), at room temperature (25°C ) to make the monomer and the cross-linking agent produce free radical polymerization, and finally add the catalyst TEMED (N, N, N', N'-Teramethylethyl...

Embodiment 2

[0212] The material for synthesizing double-ion charge-biased blood cell screening is a copolymer composed of double-ion building blocks (SBMA) and charged building blocks (positively charged building blocks (TMA) or negatively charged building blocks (SA)): SpTMAq( Where p and q represent the molar ratio of S and TMA respectively, for example, S8TMA2 represents that the molar ratio of SBMA and TMA is 8:2, the same expression is also used below) or SpSAr (wherein p and r represent the molar ratio of S and SA respectively , For example, S8SA2 means that the molar ratio of SBMA and SA is 8:2, and the same representation will be used below).

[0213] SBMA (2-(Methacryloyloxy)ethyl]dimethyl(3-sulfopropyl)-ammonium hydroxide) was mixed with TMA ([2-(Methacryloyloxy)ethyl]trimethylammonium chloridesolution) or SA(3-Sulfopropyl methacrylate potassium salt) according to different ratios and Stir evenly, then make the raw material of the monomer unit (i.e. the mixture of SBMA and TMA o...

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Abstract

The present invention relates to a double-ion charge offset-based blood cell screening material and a method for removing leukocytes from a blood sample, wherein a copolymer composed of a dual-ion construction unit and a charging construction unit is adopted as a double-ion charge offset-based material for screening blood cells. The dual-ion construction unit at least comprises a positively charged group and a negatively charged group, wherein the distance between the positively charged group and the negatively charged group is one to five the length of a carbon bond.

Description

technical field [0001] The present invention relates to a double ion charge deviation type material and a method for removing white blood cells from a blood sample, in particular to a double ion charge deviation type blood cell screening material and a method for passing a blood sample through a double ion A method of removing leukocytes from a blood sample by means of a filter material made of a charge-biased blood cell screening material. Background technique [0002] Usually, leukocytes can be filtered out of blood by a filter containing fibrous material, and since platelets and leukocytes are negatively charged, the surface of the filter material is generally treated to be positively charged, except for the sponge structure (porosity) of the filter material , using the positively charged filter surface to adsorb negatively charged platelets and white blood cells. In addition, in order to avoid coagulation or activate platelets, anticoagulants need to be added to the blo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D39/16B01J20/26C08F220/38C08F220/34
Inventor 张雍钟政峰陈圣翰林文琳
Owner CHUNG YUAN CHRISTIAN UNIVERSITY
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