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Gene detection method for transcriptional regulation related gene of spermatoblast

A technology for transcriptional regulation and gene detection, applied in the field of molecular biology, can solve problems such as spermatogenesis obstacles, affecting gene transcriptional activity, and reducing the effect of

Inactive Publication Date: 2015-05-20
上海中优医药高科技股份有限公司
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Problems solved by technology

The combination of transcription repressor protein and promoter will affect the gene transcription activity, cause the sensitivity of androgen receptor to decrease, and the combination of androgen receptor complex will decrease, which will lead to the decrease of androgen effect and cause spermatogenesis disorder

Method used

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  • Gene detection method for transcriptional regulation related gene of spermatoblast
  • Gene detection method for transcriptional regulation related gene of spermatoblast
  • Gene detection method for transcriptional regulation related gene of spermatoblast

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Embodiment Construction

[0084] The purpose of the present invention is to provide a method for detecting genes related to sperm cell transcriptional regulation, and to analyze the risk of male infertility in combination with environmental exposure factors and lifestyle influencing factors related to azoospermia and oligospermia unique to the Chinese population. Predictive evaluation.

[0085] To achieve the above objects, the present invention provides a method for detecting genes related to sperm cell transcription regulation. The specific steps are:

[0086] Step 1: Types of samples tested and sampling methods

[0087] Scraping the left and right cheeks in the oral cavity with a sampling swab requires at least 40 scrapes up and down each to ensure that a sufficient amount of cell samples are collected;

[0088] Step 2: Extraction method of genomic DNA

[0089] The genomic DNA of each oral epithelial cell sample was extracted by silica gel adsorption, and the time was 2 hours to 2.5 hours. After ...

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Abstract

The invention discloses a gene detection method for a transcriptional regulation related gene of spermatoblast. The method is characterized by comprising the following steps: providing transcriptional regulation related gene detection of the spermatoblast to all subjects by simultaneously detecting and analyzing SNPs locus gene carrying modes of a NOS3 gene and a FASLG gene of an individual; and combining special azoospermia and oligospermia related environmental exposure factors and life style influence factors of Chinese groups to predict and evaluate the onset risk of male infertility.

Description

technical field [0001] The invention relates to a method for detecting genes related to sperm cell transcriptional regulation, which can be used to evaluate the detection of genes related to sperm cell transcriptional regulation, and belongs to the technical field of molecular biology. Background technique [0002] The most important thing for men to perform their reproductive functions is the quality of their sperm. Azoospermia is the most common form of male infertility, accounting for about 15%-20% of male infertility patients. Azoospermia not only seriously affects male fertility, but also brings many negative problems to male psychology and normal family life. At present, the deletion, mutation and genetic polymorphism of spermatogenesis-related genes are considered to be important genetic causes of male spermatogenesis disorders. [0003] Azoospermia is a complex disease affected by both genetic and environmental factors, and the pathogenesis may involve multiple gen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q1/6858C12Q2600/156C12Q2600/172C12Q2561/101C12Q2563/107
Inventor 毛丹丹王校张奕
Owner 上海中优医药高科技股份有限公司
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