Aldosterone detection kit as well as preparation method and application thereof
An aldosterone and kit technology, applied in the field of detection kits, can solve the problems of radiation damage, narrow detection range, long time consumption, etc.
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Embodiment 1
[0059] (1) Labeling of ALD antigen, the specific steps are as follows:
[0060] Preparation of dialysate (F solution): Add Na to a 5000ml container 2 CO 3 14.31g and NaHCO 3 26.46g, add purified water to dilute to 4500ml, and place the prepared F solution on a magnetic stirrer for later use.
[0061] Choose a dialysis bag with a suitable cutoff (commonly used molecular weight of 14000), measure the size enough to accommodate solution F, tie one end tightly after wetting, and test for leaks with purified water 3 times (no leakage is required).
[0062] Take 100 μg of ALD antigen and adjust to 1 ml with 0.1 mol / L pH9.5 carbonic acid buffer (F solution). Put it into the dialysate, stir and dialyze at room temperature for 2 hours, add 300 μg of ABEI activated ester to the dialyzed solution, and react at 37°C for 2 hours.
[0063] The ligation product of ALD antigen and ABEI was purified by G-25 gel column.
[0064] D. 2 Solution preparation: Add 200ml of 0.5M phosphate buffe...
Embodiment 2
[0081] (1) Labeling of ALD antigen, the specific steps are as follows:
[0082] Preparation of dialysate (F solution): Add Na to a 5000ml container 2 CO 3 14.31g, NaHCO 3 26.46g, add purified water to dilute to 4500ml. The prepared F solution was placed on a magnetic stirrer for later use.
[0083] Choose a dialysis bag with a suitable cutoff (commonly used molecular weight of 14000), measure the size enough to accommodate solution F, tie one end tightly after wetting, and test for leaks with purified water 3 times (no leakage is required).
[0084] Take 100 μg of ALD antigen and adjust to 1 ml with 0.1 mol / L pH9.5 carbonic acid buffer (F solution). Put it into the dialysate, stir and dialyze at room temperature for 2 hours, add 300 μg of ABEI activated ester to the dialyzed solution, and react at 37°C for 2 hours.
[0085] The ligation product of ALD antigen and ABEI was purified by G-25 gel column.
[0086] D. 2 Solution preparation: add 200ml 0.5M phosphate buffer sa...
Embodiment 3
[0108] (1) labeling of anti-ALD antibody, the specific steps are as follows:
[0109] Preparation of dialysate (F solution): Add Na to a 5000ml beaker 2 CO 3 14.31g, NaHCO 3 26.46g, add purified water to dilute to 4500ml, and place the prepared F solution on a magnetic stirrer for later use.
[0110] Select a dialysis bag with a suitable cutoff (commonly used molecular weight of 14000), measure the size enough to accommodate solution F, tie one end tightly after wetting, and test for leaks with purified water 3 times (no leakage is required).
[0111] Take 1 mg of anti-ALD antibody and adjust it to 1 ml with 0.1 mol / L carbonic acid buffer (F solution) at pH 9.5. Put it into the dialysate, stir and dialyze at room temperature for 2 hours, add 300 μg of ABEI activated ester to the dialyzed solution, and react at 37°C for 2 hours.
[0112] The ligation product of anti-ALD antibody and ABEI was purified by G-25 gel column.
[0113] Prepare D by the method in embodiment 1 2 s...
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