Leaf micro-cuttage propagation technology for rare and endangered plant Begonia coptidifolia

A technology of spring and autumn leaves, which is applied in the field of micro-transplantation of leaves, can solve the problems of low seed germination rate, inability to meet the requirements of rapid propagation of rare and endangered plants, expanding planting area, and difficulties in seed collection

Inactive Publication Date: 2015-05-27
SHANGHAI CHENSHAN BOTANICAL GARDEN
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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem of the rare and endangered plant "Yangchun begonia" in the prior art ( Begonia coptidifolia ) relies on seeds to reproduce, it is very difficult to collect seeds, and the germination rate of seeds is very low, which cannot meet the problem of rapid propagation and expansion of rare and endangered plants themselves. A rare and endangered plant "Yangchun Begonia" ( Begonia coptidifolia ) leaf microtransplantation technology

Method used

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  • Leaf micro-cuttage propagation technology for rare and endangered plant Begonia coptidifolia
  • Leaf micro-cuttage propagation technology for rare and endangered plant Begonia coptidifolia
  • Leaf micro-cuttage propagation technology for rare and endangered plant Begonia coptidifolia

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Embodiment Construction

[0015] A rare and endangered plant "Yangchun begonia" ( Begonia coptidifolia ) leaf microtransplantation technology, is characterized in that, comprises the following steps:

[0016] Step 1, select the mature and disease-free Begonia versicolor ( Begonia coptidifolia ) leaves as cutting material;

[0017] Step 2: Cut the leaves into square cuttings with a sterilized scalpel blade, and soak them in an equal volume mixture of 800 times diluted 70% thiophanate-methyl WP and 800 times diluted 50% carbendazim WP solution for ten minutes sterilization;

[0018] Step 3, cutting: use a clean, transparent top seedling box or other airtight container to hold the cutting substrate in the greenhouse, insert the cuttings obliquely or flat on the surface of the cutting substrate, cover the cutting with a plastic cover or film, cover it, and check regularly Spray clean water to maintain suitable humidity; step 4, potting: put the cuttings that have formed a root system and grow seedlings ...

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Abstract

The invention discloses a leaf micro-cuttage propagation technology for the rare and endangered plant Begonia coptidifolia. According to the invention, innovation on the size of a cutting is realized, a propagation coefficient is greatly improved, and the technology is the first research report about leaf cuttage propagation of the rare and endangered plant Begonia coptidifolia; the technology can be carried out in an appropriate temperature range of 20 to 30 DEG C; perlite, vermiculite or other matrixes are used for leaf micro-cuttage propagation of the rare and endangered plant Begonia coptidifolia in China; and a cutting with a size of as small as 0.5 cm can undergo normal differentiation and development and grow into a seedling. The technology makes a breakthrough to the conventional concept about the size limit of cuttings, greatly improves the propagation coefficient, is improvement and innovation of asexual reproductive technology and is the first research report about leaf cuttage propagation of the rare and endangered plant Begonia coptidifolia. The technology is also applicable to begonia and other plant species suitable for leaf cuttage propagation.

Description

technical field [0001] The invention relates to a rare and endangered plant "Yangchun begonia" ( Begonia coptidifolia ) leaf microtransplantation technology. Background technique [0002] Yangchun begonia ( Begonia coptidifolia ) is the only species of multi-reverted leaves among domestic begonias, which has very important scientific research value. The distribution of this species is very narrow and limited, and it is only distributed in a small amount on some stones of several Qinggou in Ehuangzhang Provincial Nature Reserve, Yangchun City, Guangdong Province. The habitat is very special and fragile, so it is listed as a national rare and endangered plant. One of the key protected species in nature reserves. Therefore, it is of great significance to carry out research on introduction, mass reproduction, ex situ conservation and natural return of this species. For this reason, we conducted research on the reproductive biology of this species for the first time in the wo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00
CPCA01G31/00A01G24/00
Inventor 田代科李湘鹏李行娟
Owner SHANGHAI CHENSHAN BOTANICAL GARDEN
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