A microscopic analysis device

Abstract

The invention provides a microscopic analysis device, which includes a laser light source device, a light transmission device, a random optical reconstruction microscopic device, an atomic force microscopic device, a sample carrying device and an information integration output device. The light source beam provided by the laser light source device is introduced into the optical reconstruction microscopic device by the light transmission device, and the optical reconstruction microscopic imaging is performed on the sample to be tested on the sample carrying device; at the same time, the atomic force microscopic device performs atomic force Microscopic imaging; the information integration output device integrates random optical reconstruction microscopic information and atomic force microscopic information to output. The method solves the problem that the existing atomic force microscopy imaging cannot qualitatively analyze samples, and can be widely used in the fields of biology, polymers and materials.

Description

technical field [0001] The invention relates to a combination technology of a stochastic optical reconstruction micro-device and an atomic force micro-device, belonging to the field of microanalysis. Background technique [0002] Optical microscope is an essential tool for biologists to do research and explore the mysteries of life. Light microscopy has been used to gain a first glimpse at the intricate structure and morphology inside cells. However, limited by the optical diffraction limit, the resolution of conventional optical microscopes is difficult to reach below 200nm. In recent years, the invention of super-resolution fluorescence microscopy breaks through the optical diffraction limit. These include stimulated emission depletion microscopy (STED), saturated structured illumination microscopy (SSIM), stochastic optical reconstruction microscopy (STORM), and photoactivated localization microscopy (PALM). The three-dimensional spatial resolution of these methods is ...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a microanalysis device, which solves the problem that the existing atomic force microscopy imaging cannot qualitatively analyze the sample, and can obtain the molecular composition information of the sample while obtaining the three-dimensional shape information of the sample, and the resolution can reach 10- 20nm, can be widely used in biomolecular sample analysis, cell membrane structure analysis, cell imaging, nanostructure analysis and nanocatalysis detection, etc.

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