Tissue culture technique of aquilaria sinensis

A technology of tissue culture and arborina, which is applied in the field of plant tissue culture, can solve problems such as unfavorable protection of high-quality germplasm resources, restrictions on the promotion and planting of high-quality arborina, and separation of offspring traits of seed reproduction, so as to achieve the effect of accelerating promotion and resource development and utilization

Inactive Publication Date: 2015-06-10
冯文杰
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Akiras chinensis is mainly propagated through seeds, but due to the separation of offspring traits of seed propagation, it is not conducive to the protection of high-quality germplasm resources, which restricts the popularization and planting of high-quality Akiras argyi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Induction of clustered buds: Select the budding stems of young trees that grow vigorously and have no diseases and insect pests in the current year as explants, soak them in an aqueous solution of detergent for 5 minutes, then rinse them with tap water for 10 minutes, dry the surface moisture and set aside. Soak in 75% ethanol for 5 seconds in an ultra-clean workbench, sterilize with 0.1% mercuric chloride solution for 8 minutes, wash with sterile water for 3 times, cut into stems with a length of 0.5-1.2 cm and inoculate them on the induction medium after drying. Cultivate in total darkness at 25°C for 15 days, then place in light for 10 hours a day, light intensity 1000x, and culture temperature 25°C for 39 days to induce the formation of clustered buds, with an induction rate of 68.5%. The induction medium is MS+2mg / L6-BA+0.5mg / L NAA+0.1mmol / L La(NO 3 ) 2 +2.0% sucrose+0.35% agar+0.05% activated carbon, the pH value is 5.8.

[0017] (2) Proliferation culture: T...

Embodiment 2

[0021] (1) Induction of clustered buds: Select the budding stems of young trees that grow vigorously and have no diseases and insect pests in the current year as explants, soak them in an aqueous solution of detergent for 8 minutes, then rinse them with tap water for 15 minutes, dry the surface moisture and set aside. Soak in 78% ethanol for 10 seconds in an ultra-clean workbench, sterilize with 0.1% mercuric chloride solution for 10 minutes, wash with sterile water for 4 times, cut into stems with a length of 0.5-1.2 cm and inoculate them on the induction medium after drying. Cultivate in complete darkness at 28°C for 18 days, then place in light for 12 hours a day, light intensity 1500x, and culture temperature 28°C for 36 days to induce the formation of clustered buds, and the induction rate is 74.9%. The induction medium is MS+4mg / L6-BA+0.9mg / L NAA+0.3mmol / L La(NO 3 ) 2 +2.0% sucrose+0.35% agar+0.05% activated carbon, the pH value is 5.8.

[0022] (2) Proliferation cultu...

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PUM

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Abstract

The invention discloses a tissue culture technique of aquilaria sinensis and relates to a seedling growing method for obtaining good-quality seedlings from the aquilaria sinensis by use of an in-vitro rapid propagation technique. According to the tissue culture technique, the tissue culture rapid propagation system of the aquilaria sinensis is established by taking the stem with buds of the aquilaria sinensis as the explant and by virtue of the processes of explant sterilization, multiple shoot induction, rooting culture, acclimatization and transplanting and the like; the tissue culture technique has important practical significance on acceleration of the popularization of the good-quality aquilaria sinensis variety and the development and utilization of resources.

Description

technical field [0001] The invention relates to a method for plant tissue culture in plant biotechnology, in particular to a technique for tissue culture of A. sinensis. Background technique [0002] Whitewood ( Aquilaria sinensis ) is the only plant source of agarwood in my country, and the supply has been in short supply for a long time. In addition, man-made predatory logging has led to the depletion of wild resources of agarwood, so it is necessary to protect its resources. [0003] In recent years, the development of Acacia sinensis resources has reached a certain scale, and GAP planting bases have been established in Guangxi, Guangdong, Fujian, etc., which have played a certain role in promoting the protection of Acacia arborica wild resources. At present, the seedlings of A. chinensis are propagated mainly through seeds, but the separation of offspring traits due to seed propagation is not conducive to the protection of high-quality germplasm resources, and restr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 梁仕华
Owner 冯文杰
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