Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Signal-transducer-and-activator-of-transcription suppressing polypeptide and application thereof

A technique of transcriptional activator and inhibitory polypeptide, which is applied in the field of relapsed and metastatic polypeptides to improve the survival rate

Inactive Publication Date: 2015-06-10
SUZHOU PULUODA BIOLOGICAL SCI & TECH
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no well-developed signal transduction and transcription activator inhibitory polypeptide, which is used to prevent and treat cancer recurrence and metastasis after breast cancer surgery

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Signal Transducer and Transcription Activator Inhibiting the Migration of Peptide Breast Cancer Cell MCF-7.

[0015] Using scratch test. First use a marker pen on the back of the 24-well plate, and use a ruler to draw horizontal lines evenly, about every 0.5~1cm, across the holes. Three lines were passed through each well; MCF-7 cells grown in logarithmic 5 Added to 24-well culture plate, cultivated for 24h. On the second day, compare the ruler with a 10 μl pipette tip, scratch perpendicular to the horizontal line on the back, and use the intersection of the scratch and the horizontal line on the back as a fixed observation point; add different concentrations of experimental wells and positive drug control wells respectively. Drug signal transduction and transcription activator inhibitory polypeptide (synthetic by Shanghai Sangon) and positive control drug vincristine; the same volume of solvent was added to the blank group, and five replicate wells were set in each w...

Embodiment 2

[0017] Signal Transduction and Transcription Activator Inhibiting Polypeptide IC50 on Growth and Survival of Human Breast Cancer Cells Cultured in Vitro.

[0018] Using the MTT colorimetric method. Human breast cancer cells growing logarithmically at 1.0×10 5 Add it into a 96-well culture plate and culture it for 24 hours. Add different concentrations of experimental drug signal transduction and transcription activator inhibitory polypeptide (Shanghai Sangong Synthetic) and positive control drug paclitaxel to the experimental wells and positive drug control wells; add the same volume of Paclitaxel to the blank group. solvent. Set up five duplicate wells per well, culture for 48h, add MTT to each well at 0h, 2h, 8h, 14h, 20h, 24h, 36h, and 48h respectively, add DMSO after 4h, incubate for 30min, and incubate at 620nm in a microplate reader. The absorbance A value was measured, according to the formula human breast cancer cell growth inhibition rate=(1-absorbance value of the ...

Embodiment 3

[0020] The in vivo activity of the signal transduction and transcriptional activator inhibitory polypeptide was tested in a tumor model.

[0021] The MCF-7 tumor model was established, and the positive control drug paclitaxel was added; the same volume of solvent was added to the blank group, and three doses of polypeptide (Shanghai Sangong Synthetic) were set in the experimental group: 0.75, 1.5, and 3 μM mg / Kg. After 21 days, the number of surviving mice was observed, and the survival rate was calculated. The results showed that the signal transduction and transcription activator inhibitory polypeptide can effectively protect mice and improve the survival rate of tumor-bearing mice, with the survival rate reaching 49.2%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of drugs, in particular to polypeptide for suppressing signal transducers and activators of transcription and preventing and treating recurrence and metastasis of a breast cancer after an operation is carried out. The sequence of the polypeptide is QLKNLYKDEANPSWPPTEQ and is a brand new sequence. By means of the polypeptide, proliferation and migration of breast cancer cells can be suppressed out of a body, the tumor-bearing mice survival rate can be increased in an in-vivo test, and the potential new drug development value is achieved.

Description

technical field [0001] The present invention relates to signal transduction and transcription activator inhibitory polypeptide and application thereof, in particular to a polypeptide capable of inhibiting signal transduction and transcription activator to prevent and treat breast cancer recurrence and metastasis after breast cancer surgery. Background technique [0002] The female mammary gland is composed of skin, fibrous tissue, mammary glands and fat. Breast cancer is a malignant tumor that occurs in the mammary gland epithelial tissue. 99% of breast cancers occur in women and only 1% in men. At present, breast cancer has become a common tumor that threatens women's physical and mental health. [0003] The mammary gland is not an important organ to maintain human life activities, and breast cancer in situ is not fatal; but because breast cancer cells lose the characteristics of normal cells, the connections between cells are loose and easy to fall off. Once the cancer c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P35/00
Inventor 罗瑞雪
Owner SUZHOU PULUODA BIOLOGICAL SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com