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Preparation method for beta-chitosan with different molecular weights

A technology of chitosan and molecular weight, applied in the field of marine chemical engineering, can solve difficult problems and achieve the effect of less reagents, less time-consuming and controllable conditions

Inactive Publication Date: 2015-06-10
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the different structures, there are also certain differences in the preparation and degradation of β-chitosan and α-chitosan. Glycans face greater difficulties

Method used

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  • Preparation method for beta-chitosan with different molecular weights
  • Preparation method for beta-chitosan with different molecular weights
  • Preparation method for beta-chitosan with different molecular weights

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take by weighing 12g beta-chitosan, molecular weight is 4717KDa, degree of deacetylation is 91.04%, is dissolved in the glacial acetic acid solution of 400mL 2% (volume concentration), stirring and dissolving until obtaining clear, homogeneous concentration is 3% (mass concentration) β-chitosan. Take multiple 100ml microwave reactors, add 50ml of the above-mentioned dissolved β-chitosan to each reactor, and then add 1.5mL of 30% hydrogen peroxide respectively, at 600W, 70°C, the stirring speed is 600r / min , respectively reacted for 10min, 15min, 20min, 25min, 30min, 40min, 60min, 80min, 100min, 180min, will obtain different reaction solutions dialyzed, lyophilized and carry out the determination of molecular weight and structure (see Figure 1A , Figure 1B , Figure 2A with Figure 2B ). The weight average molecular weight (Mw) and number average molecular weight (Mn) of β-chitosan degradation products were analyzed by high performance gel exclusion chromatography (...

Embodiment 2

[0031] Weigh 12g of β-chitosan with a molecular weight of 4717KDa and a degree of deacetylation of 91.04%, dissolve it in 400ml of glacial acetic acid solution with a volume concentration ratio of 2%, stir and dissolve until a clear, homogeneous concentration of 3% β-chitosan is obtained. Chitosan. Get a plurality of 100ml microwave reactors, add 50ml of the above-mentioned dissolved β-chitosan in each reactor, and then add different amounts of 30% hydrogen peroxide to the reactors successively, and the different amounts of 30% hydrogen peroxide is 0.5 mL, 1mL, 1.5mL, 3mL, 4mL, 5mL, 10ml, 600W, 70℃, 700r / min. After reacting for different times, the obtained solutions were dialyzed with dialysis bags for 2-3 days to remove salt ions, rotated under high pressure at 50°C, and then freeze-dried under reduced pressure, and the molecular weight and structure were determined (see Figure 1A , Figure 1C , Figure 2A with Figure 2C). The weight average molecular weight (Mw) and n...

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Abstract

The invention belongs to the thalassochemistry engineering technology, and particularly relates to a preparation method for beta-chitosan with different molecular weights. Beta-chitosan is dissolved by acetic acid, the beta-chitosan with the high molecular weight and the high viscosity is degraded by the adoption of a microwave assisting method under the effect of hydrogen peroxide, and the beta-chitosan with different molecular weights is obtained. The method is easy to implement, efficient and good in repeatability, can be widely applied in industrial and related industry production and lays a theoretical foundation for beta-chitosan development and application.

Description

technical field [0001] The invention belongs to marine chemical engineering technology, and in particular relates to a preparation method of β-chitosan with different molecular weights. Background technique [0002] β-chitosan mostly exists in the cartilage of molluscs, which is different from the helical structure of α-chitosan, and is a space-parallel structure. It has good biological activity, biological safety, and chemical modifiability. Therefore, following α-chitosan, the development and application of β-chitosan has received extensive attention in recent years, and it is effectively used in many aspects such as medicine, food, and industrial flocculation. However, the molecular weight of naturally prepared β-chitosan is generally high, mostly above 2000KDa, and its solubility is difficult and its viscosity is relatively high. This is greatly related to the high molecular weight of its raw materials, which hinders the application and development of food, agriculture,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 邢荣娥何晓飞李克成秦玉坤刘松李鹏程
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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