Glioma tumor marker and application thereof

A tumor marker and glioma technology, which is applied in the direction of sugar derivatives, measuring devices, and material analysis by electromagnetic means, can solve the problems of incomplete resection and unsatisfactory clinical chemotherapy treatment effect of glioma

Active Publication Date: 2015-06-10
杭州中赢生物医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the invasive growth of glioma, it cannot be completely removed during surgery, which determines that chemotherapy is the most important part of adjuvant therapy after glioma surgery, but the effect of glioma on many chemotherapy drugs Drug resistance leads to unsatisfactory therapeutic effects of clinical chemotherapy for glioma

Method used

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  • Glioma tumor marker and application thereof
  • Glioma tumor marker and application thereof
  • Glioma tumor marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Collect serum samples or tumor tissue samples from patients with clinical glioma, grind the tissue or mix the serum into a glass test tube, add 2 mL of a mixed solvent of chloroform:methanol=1:1 (v / v), and use a dropper Gently mash the tissue, sonicate for 1h, after the sonication is over, centrifuge (1500rpm, 5min) to get the supernatant, and repeat the extraction 4 times. Then change to isopropanol: n-hexane: water = 55:25:20 (v / v / v) 2mL extraction solution for extraction, ultrasonication for 1h, after the ultrasonication is over, centrifuge to take the supernatant, and repeat the extraction 4 times. The above-mentioned extraction supernatants were combined, dried in a rotary vacuum dryer, and the dried product was stored in a low-temperature refrigerator at -20°C for later use. Next, separate acids and neutral glycosphingolipids through a sephadex A25 chromatography column, elute the sample with 0.8M sodium acetate methanol solution, collect the eluent to obtain the ...

Embodiment 2

[0031] Glycosphingolipids were extracted from 5 normal brain tissue samples (control), 6 meningioma tissue samples, 16 pituitary tumor samples and 14 glioma samples obtained from the First People's Hospital Affiliated to Soochow University, and acidified Mass spectrometry analysis of glycosphingolipids (as in Example 1). Denote the relative abundance of the total ganglioside as M (total), and the relative abundance of the three disialylated gangliosides GD1, GD2, and GD3 as M (GD), then the disialylated neurons The content of ganglioside in the tissue can be expressed as R=M(GD) / M(total)*100%. The R value in each group of samples is subjected to Student-Newman-Keuls test for statistical analysis, and the results obtained are as follows: image 3 As shown, the level of disialylated gangliosides in glioma was significantly higher than that of normal controls, pituitary tumor group and meningioma group, which indicated that disialylated gangliosides were regarded as glial tumors...

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Abstract

The invention relates to a glioma tumor marker and an application thereof. The glioma tumor marker is disialyl ganglioside, and the structure of the marker is represented by one or more of NeuAc-alpha3-Gal-beta3-GalNAc-beta4(NeuAc-alpha3)-Gal-beta4-Glu-Cer, Gal-beta3-GalNAc-beta4(NeuAc-alpha8-NeuAc-alpha3)-Gal-beta4-Glu-Cer, GalNAc-beta4(NeuAc-alpha8-NeuAc-alpha3)-Gal-beta4-Glu-Cer and NeuAc-alpha8-NeuAc-alpha3-Gal-beta4-Glu-Cer; wherein NeuAc represents sialic acid, Gal represents galactose, GalNAc represents galactosamine, Glu represents glucose and Cer represents N-fatty acyl-sphingosine. The marker can be used for clearly characterizing glioma tumors.

Description

technical field [0001] The invention relates to the technical field of tumor markers, in particular to a glioma tumor marker and its application. Background technique [0002] Glioma is one of the most common tumors occurring in humans, with a high degree of progression. Generally, from the time of diagnosis, the patient's life expectancy is not more than one year. As one of the most common tumors in central system diseases, glioma accounts for about 30%-50% of adult intracranial tumors, and has the characteristics of easy recurrence and difficult treatment. Currently, the main treatment for glioma is surgery, supplemented by radiotherapy and chemotherapy. Due to the invasive growth of glioma, it cannot be completely removed during surgery, which determines that chemotherapy is the most important part of adjuvant therapy after glioma surgery, but the effect of glioma on many chemotherapy drugs Drug resistance leads to unsatisfactory therapeutic effects of clinical chemoth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62C07H15/10
Inventor 吴忠福李云森许秀坤
Owner 杭州中赢生物医疗科技有限公司
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