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Pectinase produced by blue mold-microzyme mixed fermentation and application thereof

A technology of mixed bacteria fermentation and pectinase, applied in the application, enzyme, enzyme and other directions, can solve the problems of poor pectin degradation, difficult to control the degradation process, and insufficient degradation.

Active Publication Date: 2015-06-17
CHINA TOBACCO HENAN IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complexity and diversity of the pectin structure in the tobacco sheet, pectinases in the prior art generally have the defects that the degradation of pectin in the tobacco sheet is not targeted, the degradation is not sufficient, and the degradation process is difficult to control. Urgent need to explore new pectinases and their application in tobacco sheet processing

Method used

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  • Pectinase produced by blue mold-microzyme mixed fermentation and application thereof
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  • Pectinase produced by blue mold-microzyme mixed fermentation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] This example mainly briefly introduces the pectinase process produced by the mixed fermentation of Penicillium and yeast and the optimization process of the related medium as follows.

[0029] The pectinase produced by the mixed fermentation of penicillium and saccharomyces is obtained by the following steps:

[0030] (1) Activation of strains , picking the microbial strains from the preservation medium, inoculating them into the activation medium, and culturing;

[0031] Described microorganism bacterial classification comprises: the preservation number that is preserved by China Industrial Microorganism Culture Collection Center (CICC) is the Penicillium violet of CICC 40923 ( Penicillium janthinellum ), referred to as Penicillium, and Pichia anomaly ( Pichia anomala ), referred to as Pichia pastoris; these two strains were purchased from the corresponding depository institutions;

[0032] The activation medium of Penicillium is PDA medium (potato 300g / L, glucose...

Embodiment 2

[0061] In this example, the crude enzyme solution prepared in Example 1 was used to degrade tobacco flakes, thereby reducing the pectin content in tobacco flakes, and further sensory evaluation and analysis were carried out. The brief introduction is as follows.

[0062] In Example 1, the application of the pectinase produced by the mixed fermentation of Penicillium and Pichia pastoris in the processing of tobacco flakes, the obtained crude enzyme liquid (theoretically, the crude enzyme liquid with any enzyme activity can be used to degrade pectinase) Gelase, this example only takes the crude enzyme solution with the highest enzyme activity as an example, that is, the enzyme activity is 3880.0U / mL, to discuss the degradation effect of pectinase and the improvement of tobacco quality) Biodegradation is carried out according to different material-liquid ratios deal with. The specific process is:

[0063] The crude enzyme liquid was evenly sprayed on 10 g of tobacco flakes with ...

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Abstract

The invention belongs to the technical field of tobacco preparation, and particularly relates to a pectinase produced by blue mold-microzyme mixed fermentation and application thereof in a tobacco sheet working process. The pectinase is prepared by the steps of strain activation, seed solution preparation, fermentation culture, enzyme solution crude extraction and the like. The tobacco sheet and pectinase in a ratio of 1:(1-10) are subjected to enzymolysis in the crude enzyme solution at 40-50 DEG C for 2-6 hours, thereby treating the tobacco sheet. The overall technical line is as follows: the specific strains are subjected to mixed fermentation, the optimal fermentation conditions are screened and optimized to obtain the crude enzyme solution with the maximum pectinase enzyme activity, and the crude enzyme solution is used for treating the tobacco sheet to lower the pectinase content, thereby obtaining the optimal degradation effect. The maximum enzyme activity of the crude enzyme solution can reach 3880 U / mL. When the crude enzyme solution is used for treating the tobacco sheet, the pectinase content in the tobacco sheet can be lowered from 2.07% to 1.48%, thereby well improving the quality of the tobacco.

Description

technical field [0001] The invention belongs to the technical field of tobacco preparation, and in particular relates to a pectinase produced by mixed fermentation of penicillium and yeast and its application in the process of tobacco sheet processing. Background technique [0002] Pectinase is a complex enzyme composed of various components, each component can synergistically complete the degradation of pectin, and has a wide range of uses in food processing, feed processing, textile, papermaking, environmental protection, etc. The production of pectinase has a history of more than 40 years, and there are mainly two production methods: solid-state fermentation method and liquid fermentation method. Many microorganisms have the ability to produce pectinase. In the prior art, pectinase-producing enzyme-producing strains are mainly fungi, and Aspergillus species are widely used. Generally speaking, the pectinase activity produced by the solid-state fermentation method is rela...

Claims

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Application Information

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IPC IPC(8): C12N9/88C12N9/26C12N9/18A24B15/20C12R1/80C12R1/84
CPCA24B15/20C12N9/18C12N9/88C12Y301/01011C12Y402/02002
Inventor 郝辉马宇平许春平孙斯文聂聪王文领
Owner CHINA TOBACCO HENAN IND
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