Brain-like tissue in-vitro printing and culture system and brain-like tissue in-vitro printing and culture method

Abstract

The invention discloses a brain-like tissue in-vitro printing and culture system and a brain-like tissue in-vitro printing and culture method. The system comprises a printing and culture module and a control module, wherein the printing and culture module comprises a mechanical hand, a printing spray head box, printing spray heads, a mobile platform and a liquid slot; the control module comprises a computer, a signal acquirer and a control box. According to the brain-like tissue in-vitro printing and culture system disclosed by the invention, an in-vitro brain tissue constructing method which is integrated with printing and culture functions can be used for increasing a survival rate of nerve cells in a printing process and later-stage tissue, preventing the interferences of environmental changes to printed brain-like tissue in a process of transferring to the culture system form the printing system, keeping the integral activity and the function construction of the tissue, the printing spray heads are combined with the mechanical hand to realize hybrid printing of various bracket materials and various cells, and a brain-like tissue gradient structure with a special function is conveniently constructed according to a relationship between each area structure of the brain tissue and the functions; the printing and culture device is provided with culture conditions for brain tissue in-vitro regeneration and a detecting device, and can be used for performing functional evaluation in a process of promoting the brain-like tissue to form.

Description

technical field [0001] The invention belongs to the field of 3D printing and biomanufacturing, and specifically relates to a system and method for in vitro printing and culturing of brain-like tissue. Background technique [0002] The brain dominates all human life activities: language, movement, hearing, vision, emotional expression, etc. It can regulate digestion, breathing, and circulation. Damage to the brain will seriously threaten health. In medicine, there are some diseases that cannot be found and cured through animal research, such as schizophrenia and epilepsy. Being able to construct a brain tissue in vitro that is structurally and functionally similar to the human brain may open up a therapeutic approach capable of curing almost all brain dysfunctions and diseases. It will also help doctors better understand how to treat severe head injuries and even provide tissue that can be implanted in the human brain. In addition, compared with ordinary computers, the huma...

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Problems solved by technology

[0004] In order to solve the problems existing in the above-mentioned prior art, the object of the present invention is to provide a system and method for in vitro printing and culturing of brain-like tissue. The construction process realizes the construction and functional evaluation of the hierarchical gradient structure of brain-like tissue, and the introduction of multi-physical field stimulation enriched in the intracranial physiological environment in the functional culture system can improve the success rate of in vitro printing of brain-like tissue and the In vitro functional construction of brain-like tissue for more effective induction

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