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Streptomyces murinus and application thereof

A technology of Streptomyces cinerea, No. 1, which is applied in the field of microorganisms, can solve the problems of affecting the ecological environment, the generation of bacterial resistance, and increasing the amount of pharmaceuticals used, and achieves good environmental safety, remarkable control effect, and good inhibitory effect. Effect

Active Publication Date: 2015-06-24
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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Problems solved by technology

[0003] At present, the prevention and control of Camellia oleifera disease is mainly chemical control, but the long-term use of chemical fungicides not only affects the quality of tea fruit, but also leads to the emergence of resistance to bacteria, and increasing the amount of pesticides used will increase production costs and pesticide residues , affecting the ecological environment and destroying the ecological balance

Method used

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  • Streptomyces murinus and application thereof
  • Streptomyces murinus and application thereof
  • Streptomyces murinus and application thereof

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Embodiment Construction

[0020] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention.

[0021] 1. Screening of actinomycetes

[0022] Soil samples were collected from mangrove forest in Wenchang, Hainan. The soil samples were air-dried naturally, and actinomycetes were isolated by gradient dilution method. Take 100uL of the soil dilution solution with a certain concentration gradient and evenly spread it on the Gaoshi No. 1 medium. After culturing at 28°C for 7 days, count and count the colony according to the characteristics of colony shape, color, size, presence or absence of aerial hyphae, and pigment production. Number the isolated actinomycete colonies. At the same time, the numbered strains were purified on the Gaussian plate, and the purified and cultured actinomycetes were cultured on the Gaussian slant medium for 7 days, and then stored at low temperature for future u...

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Abstract

The invention provides streptomyces murinus and application thereof. The streptomyces murinus is named streptomyces murinus FXQ-5B, is preserved in the China Center for Type Culture Collection on October 16, 2014, and has a preservation number of CCTCC M2014487. The streptomyces murinus FXQ-5B can be used for effectively inhibiting growth and spore germination of camellia oleifera leaf blight pathogen, has the advantages of remarkable prevention effect, no pollution and good environment safety, and has an excellent effect for inhibiting other bacteria, such as fusarium oxysporum, lasiodiplodia theobromae, diaporthe phaseolorum, bipolaris setariae, pestalotiopsis menezesiana and the like. The streptomyces murinus has broad-spectrum anti-disease, and has an important and profound significance for prevention and treatment of camellia oleifera and other diseases.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a Streptomyces grinotis and application thereof. Background technique [0002] Camellia oleifera (Camellia oleifera Abel) belongs to the evergreen small trees or shrubs of Camellia genus Camellia, which is a unique woody oil tree species in my country. Camellia oil has high medicinal, edible, ecological and economic value, and is one of the most precious health-care plant edible oils in the world. my country is a country rich in camellia oleifera resources, with an existing Camellia oleifera cultivation area of ​​34 million mu, mainly distributed in the Yangtze River Basin and 18 provinces (autonomous regions, municipalities) south of it. Hainan Province has an area of ​​about 20,000 mu of camellia oleifera, mainly distributed in central Hainan areas, of which Wuzhishan City accounts for 80% of the area of ​​Camellia oleifera in Hainan. With the country's emphasis on th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/06A01N63/00A01N63/02A01P3/00C12R1/465
CPCA01N63/00A01N63/10C12N1/205C12R2001/465
Inventor 牛晓庆朱辉覃伟权陈良秋余同玉
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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