A method for identifying aliphatic chain isomers α-ketoglutaric acid and 1,3-acetone dicarboxylic acid

A technology of isomers and acetone dicarboxylic acid, which is applied in the field of discrimination and identification, can solve the problems of rare identification methods and difficulty in distinguishing, and achieve the effects of easy operation and observation, high accuracy and simple equipment

Inactive Publication Date: 2017-03-22
ANHUI UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since α-ketoglutaric acid and 1,3-acetone dicarboxylic acid have the same molecular formula and similar structure, some physical and chemical properties are also similar, and the appearance of the two is also very similar, making it difficult to distinguish between the two
At present, the content of α-ketoglutaric acid is usually determined by instrumental analysis methods such as reversed-phase high-performance liquid chromatography (RP-HPLC) and reversed-phase ion-pair chromatography (IPC), or by paper chromatography (PC). Analysis and identification; but there are few reports on the determination or identification method of 1,3-acetone dicarboxylic acid and the identification method between α-ketoglutaric acid and 1,3-acetone dicarboxylic acid

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for identifying aliphatic chain isomers α-ketoglutaric acid and 1,3-acetone dicarboxylic acid
  • A method for identifying aliphatic chain isomers α-ketoglutaric acid and 1,3-acetone dicarboxylic acid
  • A method for identifying aliphatic chain isomers α-ketoglutaric acid and 1,3-acetone dicarboxylic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] This example verifies the feasibility of the method for identifying aliphatic chain isomers α-ketoglutaric acid and 1,3-acetonedicarboxylic acid according to the following steps:

[0054] (1) Prepare the solution

[0055] First prepare the sulfuric acid of 0.025mol / L with 98% vitriol oil and distilled water as stock solution, then prepare respectively the potassium iodate solution of 0.14mol / L, the [NiL] of 0.0173mol / L with the sulfuric acid solution of 0.025mol / L ( ClO 4 ) 2 solution, 2mol / L malonic acid solution, 4mol / L hydrogen peroxide solution; add 14.5mL 0.025mol / L sulfuric acid solution, 6.5mL 0.14mol / L potassium iodate solution, 1.5mL 0.0173 mol / L[NiL](ClO 4 ) 2 solution, 3.5mL 2mol / L malonic acid solution and 14mL 4mol / L hydrogen peroxide solution to ensure that "H 2 SO 4 -KIO 3 -[NiL](ClO 4 ) 2 -MA-H 2 o 2 "The concentration of each component in the nonlinear chemical oscillation system is sulfuric acid 0.025mol / L, potassium iodate 0.02275mol / L, [Ni...

Embodiment 2

[0065] This example verifies the feasibility of the method for identifying aliphatic chain isomers α-ketoglutaric acid and 1,3-acetonedicarboxylic acid according to the following steps:

[0066] (1) Prepare the solution

[0067] First prepare 0.025mol / L sulfuric acid with 98% concentrated sulfuric acid as a stock solution, then prepare 0.14mol / L potassium iodate solution and 0.0173mol / L [NiL](ClO 4 ) 2 solution, 2mol / L malonic acid solution, 4mol / L hydrogen peroxide solution; add 13.5mL 0.025mol / L sulfuric acid solution, 6.5mL 0.14mol / L potassium iodate solution, 2mL 0.0173mol / L[NiL](ClO 4 ) 2 solution, 3.5mL 2mol / L malonic acid solution and 14.5mL 4mol / L hydrogen peroxide solution to ensure that "H 2 SO 4 -KIO 3 -[NiL](ClO 4 ) 2 -MA-H 2 o 2 "The concentration of each component in the nonlinear chemical oscillation system is sulfuric acid 0.025mol / L, potassium iodate 0.02275mol / L, [NiL](ClO 4 ) 2 8.65×10 -4 mol / L, malonic acid 0.175mol / L, hydrogen peroxide 1.45mo...

Embodiment 3

[0075] This example verifies the feasibility of the method for identifying aliphatic chain isomers α-ketoglutaric acid and 1,3-acetonedicarboxylic acid according to the following steps:

[0076] (1) Prepare the solution

[0077] First prepare 0.025mol / L sulfuric acid with 98% concentrated sulfuric acid as a stock solution, then prepare 0.14mol / L potassium iodate solution and 0.0173mol / L [NiL](ClO 4 ) 2 solution, 2mol / L malonic acid solution, 4mol / L hydrogen peroxide solution; add 15.5mL 0.025mol / L sulfuric acid solution, 6mL 0.14mol / L potassium iodate solution, 1.5mL 0.0173mol / L[NiL](ClO 4 ) 2 solution, 3mL 2mol / L malonic acid solution and 14mL 4mol / L hydrogen peroxide solution to ensure that "H 2 SO 4 -KIO 3 -[NiL](ClO 4 ) 2 -MA-H 2 o 2 "The concentration of each component in the nonlinear chemical oscillation system is sulfuric acid 0.025mol / L, potassium iodate 0.021mol / L, [NiL](ClO 4 ) 2 6.4875×10 - 4 mol / L, malonic acid 0.15mol / L, hydrogen peroxide 1.4mol / L;...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for identifying aliphatic chain isomers α-ketoglutaric acid and 1,3-acetone dicarboxylic acid, which is characterized in that: using "H2SO4-KIO3-[NiL](ClO4) 2‑MA‑H2O2” nonlinear chemical oscillation system is used as the identification solution, and the identification of aliphatic chain isomers can be realized according to the oscillation response of the aliphatic chain isomers to the system; L in [NiL](ClO4)2 is 5,7,7,12,14,14-hexamethyl-1,4,8,11-tetraazatetradec-4,11-diene. The oscillation spectrum provided by this identification method is more intuitive, not only can easily and quickly identify the aliphatic chain isomers α-ketoglutaric acid and 1,3-acetone dicarboxylic acid, but also can be widely used In other isomers, and the equipment is simple, high accuracy, easy to operate and observe.

Description

technical field [0001] The present invention relates to a kind of distinguishing and identifying method, specifically a kind of nickel complex [NiL](ClO 4 ) 2 A method for the identification of the aliphatic chain isomers α-ketoglutarate and 1,3-acetonedicarboxylic acid by a catalytic nonlinear chemical system. [0002] technical background [0003] α-Ketoglutaric acid and 1,3-acetone dicarboxylic acid have the same molecular formula, are aliphatic chain isomers, and play a pivotal role in their respective fields. As one of the intermediate products of the tricarboxylic acid cycle in the living body, α-ketoglutarate is not only widely used to determine the substrates of aminotransferases and dehydrogenases and supporting reagents for the determination of liver function, but also can be used in organic synthesis Intermediate, in addition, it is also an important additive in medicine, health products, and food; and 1,3-acetone dicarboxylic acid is one of the important pharmac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26
Inventor 胡刚张宇胡娟娟胡彦旸刘婷婷胡林宋继梅
Owner ANHUI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap