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Millettia specisoa tissue culture rooting method

A technique of tissue culture and Niu Dali, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., to achieve the effect of easy rooting operation, solving difficult rooting problems, and strong practicability

Active Publication Date: 2015-07-01
涂湘炎
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention provides a more effective, fast and low-cost rooting method aiming at the problem of difficult rooting in the current tissue culture process of cattle vigorously. The method can effectively improve the rooting rate, reduce waste of resources and time consumption in the tissue culture process, and provide Large-scale tissue culture production provides favorable conditions, and the technical scheme adopted in the present invention is as follows:

Method used

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  • Millettia specisoa tissue culture rooting method

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Effect test

Embodiment 1

[0030] Insert 3-5cm high robust aseptic seedlings into the rooting medium, the rooting medium is MS basic medium + 0.2mg.L-1 yttrium nitrate, the time of rooting culture is 15-35 days, and the root length of rooting culture is The healthy root of 2~5cm, rooting culture condition is, and culture temperature is 23~27 ℃, and illuminance is 1500~2000lx, and illumination time 8~12h / d; %above.

Embodiment 2

[0032] Insert 3-5cm high robust aseptic seedlings into the rooting medium. The rooting medium is MS basic medium + 0.7mg.L-1 yttrium nitrate. The time for rooting culture is 15-35 days. 2~5cm strong root, the rooting culture condition is, culture temperature is 23~27 ℃, and illuminance is 1500~2000lx, and illumination time is 8~12h / d; More than 92%.

Embodiment 3

[0034] Insert 3-5cm high robust aseptic seedlings into the rooting medium, the rooting medium is MS basic medium + 1.0mg.L-1 yttrium nitrate, the time of rooting culture is 15-35 days, and the root length of rooting culture is 2~5cm strong root, the rooting culture condition is, culture temperature is 23~27 ℃, and illuminance is 1500~2000lx, and illumination time is 8~12h / d; More than 97%.

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Abstract

The invention relates to the field of the agricultural planting technology and in particular relates to a millettia specisoa tissue culture rooting method. The method is carried out by four stages including germinating a millettia specisoa seed; proliferating a seedling; strengthening the seedling and rooting; the method mainly comprises the following steps: (a) sterilizing a mature millettia specisoa seed; (b) inoculating a millettia specisoa seeding into a proliferation medium to be cultivated; (c) transferring the proliferated aseptic seedling into a sound seedling culture medium to be cultivated; and (6) inoculating the sound aseptic seedling with the height of 3-5cm into MS base medium added with yttrium nitrate with the concentration of 0.2-2.5mg.L<-1> to prepare the rooting medium. According to the tissue culture rooting method, the rare earth element-yttrium (yttrium nitrate) is utilized, the formation of the tissue culture seedling callus is avoided, and the millettia specisoa tissue culture seedling rooting can be promoted, therefore, the rooting rate of the millettia specisoa is effectively improved and the production cost can be reduced greatly.

Description

technical field [0001] The invention relates to the technical field of agricultural planting, in particular to a method for vigorously tissue-cultured rooting of cattle. Background technique [0002] The botanical name of Niu Dali is Millettia speciosa Cham p., which is a wild and precious medicinal material distributed in tropical and subtropical regions. , chronic bronchitis, chronic hepatitis, leucorrhea, etc. Commonly known as mountain lotus root (Guangdong, Guangxi), blood vine, Nine Dragons Beads, Sweet Niu Dali, Niu Gu Dali (Guangxi), Dali Potato (Guangdong), etc. Niu Dali is sweet and flat in nature, has the functions of nourishing deficiency and moistening lungs, strengthening muscles and activating collaterals It has been clinically proven to have a certain curative effect on chronic diseases such as lumbar muscle strain, rheumatoid arthritis, tuberculosis, and chronic bronchitis. As early as the 1970s, Niu Dali was used in the production of Chinese patent medici...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 涂湘炎
Owner 涂湘炎