Simple and convenient method for extracting blood glucose-reducing constituents from Hippophae rhamnoides leaves

A seabuckthorn leaf and hypoglycemic technology, which is applied in the directions of medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, pharmaceutical formulations, etc., can solve difficult separation, complex components, and unsatisfactory hypoglycemic effect of extracts and other problems, to achieve the effect of strong hypoglycemic effect and excellent hypoglycemic effect

Active Publication Date: 2015-07-01
SHANXI JINKEHAI BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2014, the pentacyclic triterpenoids in seabuckthorn were determined for the first time. Due to the complex composition of this kind of substances, the components of some analogues are not easy to separate, so the content is based on the amount of corosolic acid, which is 0.05% in seabuckthorn , the content is low, so far there is no report on its enrichment
The hypoglycemic effect of this level of extract is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A simple method for extracting hypoglycemic components from seabuckthorn leaves, according to the following steps:

[0020] 1. Pre-processing

[0021] After the seabuckthorn leaves are cleaned and dried, they are crushed into a coarse powder, and water 15 times the weight of the coarse powder is added to boil, cooled to room temperature, and the sea buckthorn leaf pre-boiled liquid is obtained;

[0022] 2. Insulation soaking

[0023] Add 3wt% Daqu according to the mass of the pre-cooking liquid, mix well, and keep warm for 3 days at 26-30°C;

[0024] 3. Drying

[0025] Filter the pre-boiled liquid after heat preservation and soaking, and dry the filter residue to obtain seabuckthorn leaf powder, which is set aside;

[0026] 4. Extraction

[0027] According to the mass ratio of ethanol extract: seabuckthorn leaf powder = 5:1, use the extract to reflux extract the seabuckthorn leaf powder at a temperature of 80-90°C and reflux twice; after the reflux extraction, recov...

Embodiment 2

[0030] A simple method for extracting hypoglycemic components from seabuckthorn leaves, according to the following steps:

[0031] 1. Pre-processing

[0032] After the seabuckthorn leaves are cleaned and dried, they are crushed into a coarse powder, and water 10 times the weight of the coarse powder is added to boil, and cooled to room temperature to obtain the seabuckthorn leaf pre-boiled liquid;

[0033] 2. Insulation soaking

[0034] Add 2wt% Daqu according to the mass of the pre-cooking liquid, mix well, and keep warm at 30-35°C for 5 days;

[0035] 3. Drying

[0036] Filter the pre-boiled liquid after heat preservation and soaking, and dry the filter residue to obtain seabuckthorn leaf powder, which is set aside;

[0037] 4. Extraction

[0038] According to the mass ratio of ethanol extract: seabuckthorn leaf powder = 5:1, use the extract to reflux extract the seabuckthorn leaf powder at a temperature of 80-90°C and reflux 3 times; after the reflux extraction, recover...

Embodiment 3

[0041] A simple method for extracting hypoglycemic components from seabuckthorn leaves, according to the following steps:

[0042] 1. Pre-processing

[0043] After the seabuckthorn leaves are cleaned and dried, they are crushed into a coarse powder, and water 20 times the weight of the coarse powder is added to boil, and cooled to room temperature to obtain the seabuckthorn leaf pre-boiled liquid;

[0044] 2. Insulation soaking

[0045] Add 5wt% Daqu according to the mass of the pre-cooking liquid, mix well, and keep warm at 32-36°C for 5 days;

[0046] 3. Drying

[0047] Filter the pre-boiled liquid after heat preservation and soaking, and dry the filter residue to obtain seabuckthorn leaf powder, which is set aside;

[0048] 4. Extraction

[0049] According to the mass ratio of methanol extract: seabuckthorn leaf powder = 5:1, use the methanol extract to reflux extract the seabuckthorn leaf powder, and reflux twice; after the reflux extraction, the methanol is recovered ...

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PUM

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Abstract

The invention discloses a simple and convenient method for extracting blood glucose-reducing constituents from Hippophae rhamnoides leaves, belonging to the technical field of extraction of plant blood glucose-reducing constituents; the technical problem of difficulty in extraction of blood glucose-reducing constituents from the Hippophae rhamnoides leaves can be solved; and the adopted technical scheme is a simple and convenient method for extracting blood glucose-reducing constituents from Hippophae rhamnoides leaves. The method comprises the following steps of: fixing and drying the Hippophae rhamnoides leaves, then, smashing the dried leaves into coarse powder, adding water in an amount of 10-20 times of that of the coarse powder, boiling up, cooling to room temperature, and thereby obtaining precooked liquid of the Hippophae rhamnoides leaves; adding 1-5wt% of yeast according to mass of the precooked liquid, mixing evenly, preserving heat for 3-5 days at 26-36 DEG C; filtering the precooked liquid soaked under the condition of heat preservation, and drying the filter residue to get the Hippophae rhamnoides leaves powder for further use; carrying out reflux extraction to the Hippophae rhamnoides leaves powder with extracting solution according to a mass ratio of 5:1 of extracting solution to the Hippophae rhamnoides leaves powder, and performing reflux for 2-3 times; recycling the extracting solution by reduced pressure after reflux extraction, and obtaining the pasty Hippophae rhamnoides leaves extract.

Description

technical field [0001] The invention discloses a convenient method for extracting hypoglycemic components from seabuckthorn leaves, belonging to the technical field of extraction of plant hypoglycemic components. Background technique [0002] Corosolic acid, also known as 2α-hydroxyursolic acid, is a pentacyclic triterpenoid acid derived from the extract of Lagerstroemia grandis. Garcia (1940) fed ordinary rabbits with dry leaves of Lagerstroemia indica and found that the blood sugar of the rabbits decreased. In 1956, he used the extract of Lagerstroemia indica leaves to conduct clinical experiments and found that it had the effect of lowering blood sugar in diabetic patients. Murakami (1993) isolated two kinds of triterpene acids (corosolic acid and maslinic acid) from the leaves of Lagerstroemia grandis, and found that corosolic acid has the effect of lowering blood sugar. Hamamoto (1999) found that mice and rabbits had obvious hypoglycemic effect after taking corosolic a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P3/10
Inventor 张小民郭海利张吉科林美珍姚莹
Owner SHANXI JINKEHAI BIOLOGICAL PROD
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