A method for removing arsenic from soil using microorganisms combined with electrokinetic remediation
An electro-dynamic remediation and microbial technology, applied in the restoration of polluted soil, etc., can solve the problem of low arsenic removal rate, and achieve the effect of simple operation, efficient enrichment or removal, and easy access.
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Embodiment 1
[0026] Example 1 Bioelectric restoration of soil simulated in laboratory to remove arsenic
[0027] (1) The microorganism used is Acidophilus Thiobacillus ferrooxidans, which was purchased from the American Standard Biological Collection, with the preservation number ATCC23270.
[0028] (2) Acidophilus ferrooxidans was inoculated in conventional 9K medium (pH = 1.7) and pre-cultured to obtain a bacterial concentration of 10 8 ~10 9 Individual / mL microbial solution of acidophilic Thiobacillus ferrooxidans;
[0029] (3) Take 10% (about 10 6 ~10 7 cells / ml) were inoculated into the medium containing 9K liquid medium, and continued to culture at 30°C;
[0030] (4) Acidophilus ferrooxidans cultured in step (3) (about 10 8 ~10 9 pcs / ml) stored at 25 degrees for later use;
[0031] (5) Soil samples are contaminated soil samples from western Guangxi. Take 20 to 40 soils 30 to 80 cm away from the soil surface, mix them evenly, mash them, pass through 10 to 20 mesh sieves, and st...
Embodiment 2
[0038] Example 2 Removal of Arsenic by Bioelectric Restoration of Land Soil
[0039] (1) Carry out strain culture as in Example 1, make acidophilus Thiobacillus ferrooxidans (about 10 8 ~10 9 pcs / ml) for future use.
[0040] (2) Electrokinetic remediation was performed on the soil in northwestern Guangxi. The arsenic content in the soil was 187.1 mg / kg, the S content was 10.2 mg / kg, the Fe content was 21.6 mg / kg, and the permeability was 450 mm / h. Lay an anti-seepage layer at a depth of 40cm from the soil surface in the electric repair area, and set a 0.04m wide, 0.3m deep, and 1m long groove at a distance of 0.3m from the soil surface as an electrode operation, and insert Yin and Yang into two adjacent grooves. Grade electrode, the electrode is made of graphite electrode, and 0.05M HNO is added to the anode tank 3 The solution makes the pH in the anode tank 1.5, and add 0.2M HNO in the cathode tank 3 The solution makes the pH in the cathode tank 1.2, and the current density...
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