Unlock instant, AI-driven research and patent intelligence for your innovation.

Micro-sized target hybrid electro-kinetic control method based on micro-fluidic chip

A microfluidic chip and micro-sized technology, applied in the direction of stress-stimulated microbial growth, etc., can solve problems such as complex operation, loss or dilution of target cells, and time-consuming sample processing

Inactive Publication Date: 2015-07-01
QILU UNIV OF TECH
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these techniques have certain limitations. On the one hand, instruments and reagents are expensive; sample processing is time-consuming and complicated; on the other hand, sample processing may cause loss or dilution of target cells, reducing the efficiency of separation and enrichment. It is difficult to play the characteristics of other electrokinetic effects, which limits the scope of application of the method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Micro-sized target hybrid electro-kinetic control method based on micro-fluidic chip
  • Micro-sized target hybrid electro-kinetic control method based on micro-fluidic chip
  • Micro-sized target hybrid electro-kinetic control method based on micro-fluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] refer to figure 1 , figure 2 and image 3 , using a hybrid electrokinetic microfluidic chip device for micro-sized target manipulation, the device includes: microfluidic chip (1), micro-injection pump (2), function waveform generator (3), microscope (4 ), CCD (5), computer (6). Wherein, the function waveform generator (3) is connected with the microfluidic chip (1); the microsyringe pump (2) is connected with the microfluidic chip (1); the microfluidic chip (1) is placed on the microscope; the microscope (4 ) is connected with CCD (5); CCD (5) is connected with computer (6).

[0026] Place the microfluidic chip on the microscope, turn on the micro-injection pump, and set the flow rate to 0.1 μL min -1 , turn on the computer, and adjust the focus knob until a clear picture appears on the computer. Inject the pre-mixed 10μm and 25μm polystyrene microsphere suspension, turn on the function waveform generator, select square wave, apply a voltage of 14V, a frequency of...

Embodiment 2

[0028] Others are the same as in Example 1, the difference is that the function waveform generator is turned on, the square wave is selected, the applied voltage is 11V, the frequency is 1MHz, and the bias voltage is 1V, and three kinds of pre-mixed polystyrene microspheres of 5 μm, 10 μm and 25 μm are injected for mixing Electrokinetic separation, after 18s, 25 μm polystyrene microspheres converge on the central electrode and flow out from the split microchannel (105), 10 μm and 5 μm polystyrene microspheres converge on the electrode gap and flow out from the split microchannel (104, 106) .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a micro-sized target hybrid electro-kinetic control method based on a micro-fluidic chip. The micro-sized target hybrid electro-kinetic control method is characterized by comprising the following steps: cleaning the surfaces of a glass substrate with deposited micro-electrodes and a PDMS substrate with prepared micro-channels by using a plasma cleaning machine, and then bonding to prepare the micro-fluidic chip; amplifying a micro target to an appropriate size by using a microscope; controlling the injection quantity of a sample and the flow velocity of the sample in the micro-channels by using a micro-injection pump; connecting the micro-injection pump with a sample feed opening by using a rubber hose; continuously shunting a separated product to a sample discharge opening by using a shunting micro-channel; applying a group of voltages, frequencies and bias voltages to a chip micro electrode by using a function waveform generator to control the directional movement of the micro target; transmitting a video signal to a computer by virtue of a CCD; and observing the separation conditions of the micro-sized target by using the computer, further calculating indexes such as separation efficiency, and finally achieving the hybrid electro-kinetic operation of the micro-sized target on the micro-fluidic chip.

Description

Technical field: [0001] The invention relates to a hybrid electrokinetic control technology for a micro-sized target, in particular to a hybrid electrokinetic control method for realizing a micro-sized target based on a microfluidic chip. Background technique: [0002] The manipulation of micro-sized targets, especially the capture, enrichment or isolation of target cells in physiological fluids, has potential significance for early diagnosis and treatment of diseases, drug design and side effect control. For example, in the research of directly capturing cancer cells from clinical samples (such as blood, urine, etc.), because the number of circulating tumor cells in peripheral blood is small, it usually needs to be captured or enriched before further analysis. The more commonly used techniques mainly enrich these cells through some physical or chemical principles to improve the detection sensitivity of circulating tumor cells. Existing enrichment techniques can be divided ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42
Inventor 刘彧田爱华高健
Owner QILU UNIV OF TECH