Method of rapidly determining fluoroquinolone type medicines in faeces of livestock and poultry
A technology of fluoroquinolones and livestock and poultry feces, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high cost of instrument use and maintenance, low sensitivity, and complicated operation, and achieve low operation and maintenance costs and high resolution High, high-sensitivity effects
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Embodiment 1
[0026] Embodiment 1: Measure the recovery rate, detection limit and quantitative limit of this method
[0027] The specific process is as follows:
[0028] 1) Select large-scale pig farms in Liaoning Province to collect pig manure samples, perform multi-point sampling and mix them, seal them in black plastic bags after collection, and immediately store them in ice boxes for refrigerated storage, and then keep them frozen and transport them back as soon as possible Laboratory, and freeze-dried;
[0029] 2) Store the freeze-dried samples away from light, grind them with a mortar before analysis, and pass through a 2mm sieve to collect the sieved samples;
[0030] 3) Four common fluoroquinolones (norfloxacin, ciprofloxacin, lomefloxacin, and enrofloxacin) were added to four groups of pig manure samples (three parallel replicate samples in each group). The standard solution, the concentration of the four compounds in the mixed standard solution were set to 0.01mg / kg, 0.1mg / kg, 1...
Embodiment 2
[0038] Embodiment 2: Contrast the accuracy and simplicity of this method and other method determinations
[0039] The specific process of this method is as follows:
[0040] 1) Select large-scale pig farms in Liaoning Province to collect pig manure samples, perform multi-point sampling and mix, seal the samples with black plastic bags, and immediately store them in ice boxes for refrigerated storage, and then keep them frozen and transport them back as soon as possible Laboratory, and freeze-dried;
[0041] 2) Store the freeze-dried sample in the dark, grind it with a mortar before analysis, and collect the sieved sample after passing through a 2mm sieve;
[0042]3) Accurately weigh three samples of 0.50g pig manure and place them in 10mL plastic centrifuge tubes, add 5mL of 50% calcium chloride hydrochloride buffer, vortex mix for 60S, ultrasonically extract for 15 minutes, and centrifuge (4500r / min) for 10 minutes , collect the supernatant;
[0043] 4) The residue was rep...
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