Method for preparing physically reproducible protein chip

Abstract

The invention discloses a method for preparing a physically reproducible protein chip. The method utilizes capillary absorption and spatial blocking processes to immobilize a protein solution to an arrayed micro-cylinder. The method solves the problem that the existing chip difficultly keeps protein activity and has a high disposable cost. The method comprises the following steps of preparing a female mold by a silicon chip lithography technology, coating the surface of the female mold with a polymerisable organic matter liquid, carrying out vacuum degassing and thermopolymerization hardening, removing a transparent and elastic protein chip substrate of the female mold to obtain an array with one order or two order arrays, wherein each one of the one order array points has one or more cylinders, and feeding the protein solution into the micro-cylinders by a capillary electrophoresis 3D sample application technology or pressure contacting so that the protein chip is obtained. Sides of the micro-cylinders are provided with slits so that in pressing, the slits are used as exhaust channels. The micro-cylinder liquid storage-type protein chip can be regenerated by physical methods such as ultrasonic cleaning and drying treatment after use so that recycle is realized.

Description

Technical field: [0001] The invention relates to a method for preparing a physically reproducible protein chip by using arrayed tiny capillary spaces to immobilize a protein solution and micro-nano magnetic beads or quantum dots. technical background: [0002] Proteins are unique active substances of living organisms, they not only serve as structural units to build living organisms, but also perform vital activities as functional units. Many proteins can specifically bind to other proteins or gene fragments, such as the binding of antibodies to antigens, the binding of ligands to receptors, the binding of enzymes to substrates, etc. The detection of harmful substances such as bacteria and viruses can be realized by using the specific binding between proteins. Protein chip is a fast and efficient detection method born under this background. A protein chip is a type of biochip. The biggest difference between it and another type of gene chip is that it needs to have the abili...

AI Technical Summary

Problems solved by technology

As far as the physical immobilization method is concerned, the technologies such as electric field and magnetic field are complex and the chip is opaque, which causes great difficulties for subsequent detection; it is simple and easy to directly spot the protein into the gel, but the disadvantage is that the diffusion of the protein will expand the matrix, The concentration is reduced, and the matrix points may also be connected; the method of drilling holes on the substrate is also feasible, but the cost of microhole processing is high, and the quality is difficult to guarantee

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