Cadmium (Cd) strongly-induced promoter CdS2 and application thereof

A promoter and induced expression technology, which is applied in the field of biotechnology and plant genetic engineering, can solve the problems of cadmium measurement interference, etc., and achieve the effects of high accuracy, increasing effect, and low detection limit

Active Publication Date: 2015-07-08
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The national standard analysis method is the atomic absorption method. Although the detection limit of this method is low, there are many coexisting ions after the

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cadmium (Cd) strongly-induced promoter CdS2 and application thereof
  • Cadmium (Cd) strongly-induced promoter CdS2 and application thereof
  • Cadmium (Cd) strongly-induced promoter CdS2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0039] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0040] Obtaining Cd-S2 Promoter Containing Restriction Sites

[0041] 1. Plant material

[0042] The mature seeds of Nipponbare were preserved by the Biotechnology Office of the Rice Institute of Anhui Academy of Agricultural Sciences.

[0043] 2. Strains and plasmids

[0044] The Escherichia coli strain used in this study was XL1-blue; Agrobacterium tumefaciens was EHA105, which was preserved in the Biotechnology Laboratory of Rice Institute, Anhui Ac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a segmented plant gene sequence. According to the segmented plant gene sequence, a cadmium (Cd) strongly-induced rice specific gene promoter CdS2 is separated, cloned and identified from Oryza sativa L cv.Nipponbare, and acquired transgenic rice is subjected to cadmium (Cd) treatment and is subjected to GUS chemical dyeing to find that each part of treated transgenic plants is significantly dyed; and moreover, by analyzing Gus gene expression quantities before and after RT-qPCR identification treatment, results show that the Gus gene expression quantity of the transgenic plants after being subjected to cadmium (Cd) treatment is 887.9 times that of the transgenic plants before being subjected to cadmium (Cd) treatment and can reach 4 times of ACTIN activity of a constitutive promoter. The promoter disclosed by the invention can be used for reflecting the cadmium pollution strength in soil according to the expression quantity of the promoter in rice, has the characteristics of simplicity in operation, low detection limit, micro sensitivity and high accuracy, can play important significance in researching environment relationships, improving ecological environments and achieving sustainable development, can also be used for actively analyzing pollution formation principles, and ensures that valuable experiences for avoidance and controllable transformation of cadmium pollution can be further provided.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a plant cadmium-inducible expression promoter and its expression and application in rice. The promoter can drive the expression of a target gene in a plant under cadmium-induced conditions in a rice transgenic regulation system, and It can be used to determine the cadmium content in soil. Background technique [0002] With the rapid development of modern industrial and agricultural production, the industrial "three wastes", the amount of agricultural chemical fertilizers, and solid waste continue to increase, resulting in a sharp increase in the content of heavy metals in the soil. The problem of heavy metal pollution is becoming more and more serious, and has become one of the main problems worldwide (Deng Xiangxiong et al., 2009). Cadmium (Cd) is one of the most toxic heavy metals. Compared with other non-esse...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/113C12N15/82A01H5/00C12Q1/68
Inventor 魏鹏程杨剑波李浩张银萍马卉李莉秦瑞英李娟
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap