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Synthetic method for self-luminous polymer microsphere

A synthesis method and polymer technology, applied in the direction of luminescent materials, chemical instruments and methods, etc., to achieve broad application prospects, stable performance, and mild reaction conditions

Inactive Publication Date: 2015-07-15
HUBEI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are only reports of chitosan and glutaraldehyde cross-linked to generate fluorescent nanoparticles

Method used

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  • Synthetic method for self-luminous polymer microsphere
  • Synthetic method for self-luminous polymer microsphere
  • Synthetic method for self-luminous polymer microsphere

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preparation example Construction

[0014] The invention provides a synthetic method of self-luminous polymer microspheres, which comprises the following steps:

[0015] A. Provide polyethyleneimine aqueous solution and emulsifier solution, and mix the two to obtain emulsion;

[0016] B. At 15-25°C, add a crosslinking agent solution to the emulsion obtained in step A, and stir to obtain an orange-red emulsion;

[0017] C. Remove the oil phase and excess crosslinking agent from the emulsion obtained in step B to obtain self-luminous polymer microspheres.

[0018] Polyethyleneimine is a water-soluble high molecular polymer. Although it is also soluble in polar organic solvents such as ethanol and acetone, if an organic solvent is used to dissolve polyethyleneimine to form its organic solution, the crosslinking agent and polyethyleneimine will Insufficient amine binding may form broken microspheres, and aqueous solutions are safer, more environmentally friendly and more cost-effective than organic solutions.

[0...

Embodiment 1

[0027] Dissolve 2mL of PEI aqueous solution with a mass concentration of 25% and a molecular weight of 1800, 2mL of liquid paraffin and 0.2g of emulsifier span-20 in a sample bottle, and emulsify at high speed for 2 minutes;

[0028] At 15-25°C, add 2 mL of glutaraldehyde aqueous solution with a mass concentration of 25% to the sample bottle dropwise, and stir magnetically to obtain an orange-red emulsion;

[0029] The orange-red emulsion was washed and centrifuged with 2 mL of isopropanol, 2 mL of anhydrous ether, and 2 mL of deionized water, respectively, to obtain self-luminescent polymer microspheres.

Embodiment 2

[0031] Dissolve 4 mL of PEI aqueous solution with a mass concentration of 25% and a molecular weight of 6000, 4 mL of liquid paraffin and 0.4 g of emulsifier span-60 in a sample bottle, and emulsify at high speed for 4 minutes;

[0032] At 15-25°C, add dropwise 4 mL of 30% isocyanate ethanol solution into the sample bottle, and magnetically stir to obtain an orange-red emulsion;

[0033] The orange-red emulsion was washed and centrifuged with 4 mL of isopropanol, 4 mL of anhydrous ether, and 4 mL of deionized water, respectively, to obtain self-luminescent polymer microspheres.

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Abstract

The invention provides a synthetic method for a self-luminous polymer microsphere. The synthetic method comprises the following steps: A, providing an aqueous polyethyleneimine solution and an emulsifier solution and mixing the two solutions so as to obtain an emulsified liquid; B, adding a cross-linking agent into the emulsified liquid obtained in the step A at a temperature of 15 to 25 DEG C and carrying out stirring so as to obtain an orange red emulsion; and C, removing an oil phase and excess cross-linking agent in the emulsion obtained in the step B so as to obtain the self-luminous polymer microsphere. The self-luminous polymer microsphere synthesized in the invention is in a regular spherical shape, has fluorescent properties and can meet requirements on biological tracing without fluorescein marks; moreover, the self-luminous polymer microsphere synthesized in the invention has the advantages of a low price, good in-vivo and in-vitro transfection effect, no immunogenicity, stable performance, no toxicity, etc.; and the synthetic method has the advantages of simple operation, low cost, mild reaction conditions and no toxicity.

Description

technical field [0001] The invention relates to self-luminous materials, in particular to a synthesis method of self-luminous polymer microspheres. Background technique [0002] Polyethyleneimine, abbreviated as PEI, is a new type of polycationic non-viral carrier, due to its low price, good transfection effect in vivo and in vitro, and has the advantages of convenient use, large-scale production and non-immunogenicity. and received widespread attention. Because the PEI polymer contains multiple amino groups, it can form a polymeric network structure to protect certain substances in the human body, such as preventing DNA from being hydrolyzed and inactivated under the acidic conditions of lysosomes, protecting DNA, and increasing transfection rates. In addition, polyethyleneimine has a strong ability to bind and adhere to cells, and has a high transduction efficiency. [0003] Traditionally, microspheres have been labeled with fluorescein to make them fluoresce for trackin...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08L79/02C09K11/06
Inventor 何瑜宋功武严丹李娜田甜
Owner HUBEI UNIV
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