Bacillus megaterium strain and its application

Abstract

A Bacillus megaterium strain and its application, said strain is Bacillus megaterium strain BM5 (Bacillus megaterium), which was preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on February 28, 2014, and the preservation number is CGMCC NO. 8866; the application of the Bacillus megaterium strain includes: collecting tofu wastewater, adjusting the pH to 7.0, placing it in a fermenter for 121°C autoclaving for 25 minutes, and inoculating the Bacillus megaterium strain BM5 with an inoculation amount of 106 cfu / mL in the fermentation tank In the tank, aerated, cultivated at 33°C for 3 days, and then fermented into a compound enzyme solution; the strain can ferment and grow with tofu wastewater as the only nutrient substance, and produce enzymes of endoglucanase and xylanase The solution provides a new source of fermentation bacteria for the production of endoglucanase and xylanase preparations.

Description

technical field [0001] The present invention relates to a strain of Bacillus megaterium and its application. Background technique [0002] In order to make full use of resources and save costs, substances with high content of cellulose and hemicellulose, such as rice bran, wheat secondary flour, cottonseed meal, straw flour, forage, etc., are often used as raw materials to replace high-priced corn, soybean, wheat and other substances. However, a high proportion of crude fiber diets can not only be directly absorbed and utilized by livestock and poultry, but also easily cause various problems such as diarrhea and slow growth of livestock and poultry. In order to improve feed quality, feed mills and farms have fully realized the importance of adding enzymes to feed, especially non-digestive enzymes such as cellulase and hemicellulase that cannot be synthesized by animals themselves. [0003] The main components of plant cell walls are composed of cellulose, hemicellulose and ...

AI Technical Summary

Problems solved by technology

Not to mention the large loss of enzymes in this process, the use of this production procedure and the long production cycle also greatly increase the production cost of the enterprise

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