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Method for improving yield of arachidonic acid through fermentation production of mortierella alpina

A technology of Mortierella alpina and arachidonic acid, applied in the field of microbial fermentation, can solve the problems of low content, not widely used, unsuitable for industrial production, etc., and achieve the effect of low cost and increased output

Inactive Publication Date: 2015-07-29
INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] ARA can be extracted from animal tissues, but it is not suitable for industrial production due to its small content and difficulty in extraction.
In addition to animal tissue extraction, it is mainly obtained from two types of organisms in industry. One is the fermentation production of Mortierella, such as Mortierella alpina ( Mortierella alpina ), Mortierella chrysogenum ( Mortierella isabellina ), Mortierella longa ( Mortierella elongata ), etc.; obtained through the cultivation of marine microalgae, such as Porphyridium coccus ( Porphyridium ), Notch edge green algae ( Porphyridium incisa ), but this method is not widely used at present, and is only in the experimental and trial production stage

Method used

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  • Method for improving yield of arachidonic acid through fermentation production of mortierella alpina
  • Method for improving yield of arachidonic acid through fermentation production of mortierella alpina
  • Method for improving yield of arachidonic acid through fermentation production of mortierella alpina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1) Preparation of oleic acid: oleic acid is sterilized through a syringe filter, using an organic filter head of 0.22 μm.

[0033] 2) Shake flask seed culture: Under sterile conditions, take an appropriate amount of Mortierella alpina spores or mycelium and spread it on PDA medium, and culture it at 25-30°C for 7 days (5-8 days are determined according to the actual growth status. determined, each time is not necessarily the same), and then scrape an appropriate amount of spores into the Erlenmeyer flask containing the shaker flask seed medium, culture on a shaking table at 27°C and a humidity of 45% for 30 hours, and the shaking table speed is 200rpm to obtain seeds; the seeds The medium is glucose 50g / L, yeast powder 20g / L, pH 6.8; the formula of PDA medium is: glucose 20g / L, potato 150g / L, agar powder 15g / L, K 2 PO 4 0.4g / L,MgSO 4 0.2g / L, pH 6.5-7.0.

[0034] 3) Shake flask fermentation culture: put the seed liquid into the Erlenmeyer flask containing the fermen...

Embodiment 2

[0047] 1) The preparation of oleic acid is the same as in Example 1.

[0048] 2) The shake flask seed cultivation method is the same as in Example 1.

[0049] 3) Shake-flask fermentation culture: For shake-flask fermentation culture, transfer the seed liquid into a triangular flask containing fermentation medium according to the inoculum amount of 10%, and cultivate it on a shaker for 8-14 hours at 25-30°C and humidity 30%-60%. day, the rotating speed of the shaking table is 180-250rpm; when fermenting for 3 days, 1%, 2%, 3% oleic acid and the fermented liquid are respectively emulsified by the surfactant lecithin, wherein the lecithin addition is 1%, and the conversion After the emulsification, continue to culture on a shaker.

[0050] There is also a control group without adding oleic acid and only adding an equal amount of lecithin, pH 7.0-7.5, and there are 3 parallel fermentation bottles for each group of experiments, and unseparated arachidonic acid oil is obtained afte...

Embodiment 3

[0055] 1) The preparation of oleic acid is the same as in Example 1.

[0056] 2) The shake flask seed culture method is the same as in Example 1.

[0057] 3) Shake flask fermentation culture: For shake flask fermentation culture, put the seed liquid into the triangular flask containing the fermentation medium according to the inoculation amount of 10%, and cultivate it on the shaker at 25-30°C and humidity 30%-60% for 8-14 day, the rotating speed of the shaker is 180-250rmp; when fermenting for 6 days, 1%, 2%, and 3% oleic acid and the fermentation broth are respectively emulsified by the surfactant lecithin, wherein the lecithin addition is 1%, and the conversion After the emulsified liquid was cultured on a shaker, there was also a control group without adding oleic acid and only adding the same amount of lecithin, pH 6.5-7.0, and each group of experiments had 3 parallel fermentation bottles, and arachidonic acid oil was obtained after the fermentation ; The fermentation me...

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Abstract

The invention discloses a method for improving the yield of arachidonic acid through fermentation production of mortierella alpina, which is used for improving the yield of arachidonic acid through adding oleic acid into a mortierella alpina fermentation medium. After the inoculation of mortierella alpina fermentation flask, different proportions of oleic acid is added at different fermentation time points and the emulsification action of an emulgator is utilized to detect the yield of arachidonic acid in a final fermentation broth, and comparison shows that the yield of arachidonic acid with oleic acid added is obviously higher than the fermentation yield of arachidonic acid without oleic acid. The method has the advantages of remarkably improving the yield of arachidonic acid, reducing the fermentation cost, pollution-free, and the like, and is suitable for commercial production.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for increasing the yield of arachidonic acid fermented by Mortierella alpina. technical background [0002] Arachidonic acid is all-cis eicosa-5,8,11,14-tetraenoic acid, abbreviated as ARA (Arachidonic acid), also known as AA, which is a long-chain polyunsaturated fatty acid of the omega-6 series. It plays an important role as a metabolic intermediate. As the direct precursor of various circulating eicosanoid derivatives such as prostaglandins and leukotrienes, ARA has multiple physiological regulatory functions; at the same time, ARA can promote the development of the nerves and brain of infants, and plays a role in improving the intelligence of infants. Crucial role. In addition, ARA also has activities such as improving visual acuity, enhancing blood vessel elasticity, reducing blood viscosity, anti-inflammation, and protecting skin. It has been widely used in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
CPCC12P7/6427
Inventor 凌瑞孙立洁陈祥松袁丽霞姚建铭王纪吴金勇刘伟伟
Owner INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
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