A kind of preparation method of high-purity hop total flavonoids
A technology for hops and total flavonoids, which is applied in the field of preparation of high-purity hops total flavonoids, can solve problems such as insufficient supply, and achieve the effects of good product color, high product yield and content, and simple steps
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Embodiment 1
[0023] First, add 2kg of raw hops (hop ear) into 20L of 60% ethanol extract, reflux at 65°C for 2h, then filter and wash to obtain 19.6L of extract; Continue to reflux for 2 hours in the concentrated ethanol extract, filter, and combine the two extracts, which is about 41.0 L. Concentrate the obtained extract until it has no alcohol smell, then dilute it with water and let it stand to cool, then filter it through diatomaceous earth, pass the filtrate through a polyamide column, and wash it with purified water and ethanol eluent with a concentration of 70% in turn Remove, and collect the effluent of the eluted part of the ethanol eluent. Dilute the effluent with water and pass it through a DM130 macroporous resin column, elute again with purified water, 25% ethanol eluent and 95% ethanol eluent, and collect 95% ethanol eluent again The effluent from the liquid elution fraction. Finally, the effluent was added to activated carbon for decolorization, then suction filtered while...
Embodiment 2
[0025] First, add 2kg of raw hops (hop ear) into 20L of ethanol extract with a concentration of 90%, reflux at 80°C for 2h, then filter and wash to obtain 18.2L of extract; Continue to reflux in the ethanol extract of equal concentration for 2 hours, repeat the extraction twice, filter, and combine the extracts three times, which is about 62.5 L. Concentrate the obtained extract until it has no alcohol smell, then dilute it with water and let it stand to cool, then filter it through diatomaceous earth, pass the filtrate through a polyamide column, and wash it with purified water and ethanol eluent with a concentration of 70% in turn Remove, and collect the effluent of the eluted part of the ethanol eluent. Dilute the effluent with water and pass through the HPD-600 macroporous resin column, elute again with purified water, 25% ethanol eluent and 95% ethanol eluent, and collect 95% ethanol again Eluent The effluent from the eluted fraction. Finally, the effluent was decoloriz...
Embodiment 3
[0027] Firstly, add 2kg of raw hops (hop ear) into 20L of ethanol extract with a concentration of 90%, reflux at 65°C for 2 hours, then filter and wash to obtain 20.3 L of extract; Continue to reflux in the ethanol extract of equal concentration for 2 hours, filter, and combine the two extracts, approximately 42.2 L. Concentrate the obtained extract until it has no alcohol smell, then dilute it with water and let it stand to cool, then filter it through diatomaceous earth, pass the filtrate through a polyamide column, and wash it with purified water and ethanol eluent with a concentration of 70% in turn Remove, and collect the effluent of the eluted part of the ethanol eluent. Dilute the effluent with water and pass it through a D101 macroporous resin column, elute again with purified water, 25% ethanol eluent and 95% ethanol eluent, and collect 95% ethanol eluent again The effluent from the liquid elution fraction. Finally, the effluent was added to activated carbon for dec...
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