DNA (deoxyribonucleic acid) fragments of ginseng specificity RAPD (random amplified polymorphic DNA) mark, and primer pair and method for identifying SCAR mark of ginseng

Abstract

The invention discloses DNA (deoxyribonucleic acid) fragments of a ginseng specificity RAPD (random amplified polymorphic DNA) mark, and a primer pair and a method for identifying an SCAR mark of ginseng. The method comprises the following steps of screening the DNA fragments of ginseng specificity by using a random amplified polymorphic DNA method; recycling and cloning the DNA fragments and measuring the sequence of the DNA fragments to obtain a specificity sequence; designing a specific primer 1 and a specific primer 2 according to the specificity sequence; transforming the RAPD mark into a stable SCAR mark; and performing amplification to obtain a specificity band type which comprises two DNA fragments of which the sizes are 300bp and 130bp respectively by a method of detecting the ginseng and by using quick molecules of the SCAR mark. The specificity band type does not exist in American ginseng, pseudo-ginseng, aizoon stonecrop herbs, panicled fameflower roots, largeleaf japanese ginseng rhizome, lobedfruit schizocapsarhizome, radix curcumae longae, panax japonicus, pokeberry roots and other researched 58 types traditional Chinese medicinal materials, and all the traditional Chinese medicinal materials are in a negative amplification state. The ginseng can be identified by the method, and samples can be identified by simple DNA extraction, PCR (polymerase chain reaction) specificity amplification and electrophoresis detection. The method has the advantages of easiness in operation, high specificity, high repeatability and the like, and can be used for quickly identifying the ginseng.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, relates to the field of identification of traditional Chinese medicines and medicinal materials, in particular to a pair of specific primers and a method for identifying ginseng using SCAR technology. Background technique [0002] Ginseng is the dried roots and rhizomes of Panax ginseng C.A.Mey., Araliaceae, Panax, which are mostly collected in autumn, washed and dried in the sun or dried. The cultivated ginseng is commonly called "garden ginseng"; the one that grows naturally in the wild state of the forest is called "forest ginseng", and is commonly called "seed sea". It is mainly produced in the three northeastern provinces of my country and North Korea. It is known as the "King of Hundred Medicines"; it has the effects of invigorating vitality, strengthening the heart, strengthening the spleen and lungs, and calming the nerves; , deficiency of the spleen, lack of food, asthma and co...

AI Technical Summary

Problems solved by technology

RAPD is a random sequence of 10 bases. Genomic DNA is amplified by PCR reaction. Since the base positions of different genomic DNA sequences have obvious differences, the binding sites of primers in different segments are also different, so the amplification The number, strength and other characteristics of the bands are different, resulting in discontinuous DNA products and obtaining more band information, so that different species show polymorphism; the advantage of RAPD is that the amount of DNA required is very small, and the operation Simple, low equipment requirements, but because the RAPD primer is only 10bp, the annealing temperature is low, and the repeatability is relatively poor

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