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Method for aseptic seed germination of swertia pseudochinensis hara

A technology for the germination of Swertia chinensis and seeds, which is applied in the field of aseptic germination of Swertia chinensis seeds, which can solve the problems of failure to germinate, small seeds, and damage to seeds, and achieve easy germination, low cost, and short germination time of seeds Effect

Inactive Publication Date: 2015-08-19
马云桐 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the seeds of Swertia pilosa are small, and when carrying out its tissue culture research, the seeds are often damaged due to excessive disinfection and cannot germinate, which has become a key factor restricting the mass production of seedlings through tissue culture.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] An example of the method for the aseptic germination of Swert chinensis seeds of the present invention comprises the following steps:

[0015] (1) Seed disinfection: Rinse the seeds of Swertia chinensis, put them into 15% (v / v) 84 disinfectant solution for immersion and disinfection for 15 minutes, and then rinse with sterile distilled water for 3 times to obtain the sterilized seeds;

[0016] (2) Aseptic germination: inoculate the sterilized seeds obtained in step (1) into MS medium for cultivation, and cultivate them for 15 days at a cultivation temperature of 23-27° C., a light intensity of 1500 lux, and a light time of 12-14 hours / day. The next day, the seeds germinated to obtain sterile test-tube plantlets, in which 30g / L sucrose and 3.4g / L agar were added to the MS medium, and the pH value of the medium was 5.8. According to statistics, the germination rate was 89%.

Embodiment 2

[0018] Another example of the method for the aseptic germination of Swert chinensis seeds of the present invention comprises the following steps:

[0019] (1) Seed disinfection: Rinse the seeds of Swertia chinensis, put them into 25% (v / v) 84 disinfectant solution for immersion and disinfection for 10 minutes, then rinse with sterile distilled water for 3 times to obtain the sterilized seeds;

[0020] (2) Aseptic germination: inoculate the sterilized seeds obtained in step (1) into MS medium for cultivation, and cultivate them for 15 days at a cultivation temperature of 23-27° C., a light intensity of 1500 lux, and a light time of 12-14 hours / day. The next day, the seeds germinated to obtain sterile test-tube plantlets, in which 30g / L sucrose and 3.4g / L agar were added to the MS medium, and the pH value of the medium was 5.8. According to statistics, the germination rate was 85%.

Embodiment 3

[0022] Another example of the method for the aseptic germination of Swert chinensis seeds of the present invention comprises the following steps:

[0023] (1) Seed disinfection: Rinse the seeds of Swertia chinensis, put them into 35% (v / v) 84 disinfectant solution for immersion and disinfection for 5 minutes, then rinse with sterile distilled water for 3 times to obtain the sterilized seeds;

[0024] (2) Aseptic germination: inoculate the sterilized seeds obtained in step (1) into MS medium for cultivation, and cultivate them for 15 days at a cultivation temperature of 23-27° C., a light intensity of 1500 lux, and a light time of 12-14 hours / day. The next day, the seeds germinated to obtain sterile test-tube plantlets, in which 30g / L sucrose and 3.4g / L agar were added to the MS medium, and the pH value of the medium was 5.8. According to statistics, the germination rate was 82%.

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Abstract

The invention discloses a method for aseptic seed germination of swertia pseudochinensis hara. The method comprises the following steps: (1) washing seeds of the swertia pseudochinensis hara, placing in 84 disinfectant of 15-35 percent (v / v) to soak and disinfect for 5-15 minutes, and then washing with sterile distilled water for three times to obtain disinfected seeds; (2) inoculating the disinfected seeds obtained by the step (1) to an MS (Murashige and Skoog) culture medium to culture, culturing for 15 days under the conditions of culture temperature of 23-27 DEG C, illumination intensity of 1,500 lux and illumination time of 12-14 hours / day, performing seed germination to obtain sterile test-tube plantlets, wherein 30 g / L of saccharose and 3.4 g / L of agar are added into the MS culture medium, and the pH value of the culture medium is 5.8. By using the aseptic germination method, the germination rate reaches 82-91 percent, the problem of difficulty in seed germination of tissue culture of the swertia pseudochinensis hara is effectively solved, and a foundation is laid for sustainable utilization of the swertia pseudochinensis hara resource.

Description

technical field [0001] The invention relates to a method for germination of plant seeds, in particular to a method for aseptic germination of Swert spinach seeds. Background technique [0002] Swertia pseudochinensis Hara is the dried whole herb of Swertia pseudochinensis Hara, a plant of the genus Swertia in the Gentianaceae family. It is commonly known as "medicine", and is mainly produced in China, North Korea, and Russia. Bitter in the mouth, cold in nature. It has the effects of clearing away heat and detoxification, promoting dampness and invigorating the stomach. Mainly used to treat jaundice hepatitis, dysentery, stomachache, indigestion, toothache, red eyes, headache, aphtha; external application can cure sores. Swertia pilosa is a traditional Mongolian medicine. The Mongolian medicine is called Chagan-Timur-Digeda or Bilachute-Digeda. It is mainly used for gallstones, jaundice, food accumulation, liver heat, injury heat, and stomach heat. , has a long history of...

Claims

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Application Information

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IPC IPC(8): A01C1/02A01C1/08
Inventor 马云桐韦坤华李彦
Owner 马云桐
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