Recombinant bacteria with improved alpha-phenylpyruvic acid transformation production efficiency

A technology of phenylpyruvate and recombinant bacteria, applied in the field of recombinant bacteria, can solve the problems of low product yield, increased difficulty of separation and purification, toxic and harmful products, etc., achieve high yield, solve cumbersome steps, and increase production
CN104862264AActive Publication Date: 2015-08-26JIANGNAN UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2015-08-26

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Abstract

The invention discloses recombinant bacteria with improved alpha-phenylpyruvic acid transformation production efficiency, and belongs to the field of biotechnology. According to the invention, the knockout of aspC, tyrB and ilvE genes from Escherichia coli is achieved successfully, so that the degradation of the product PPA by cells is blocked, the yield of extracellularly transformed L-phenylalanine is further improved, and the yield of PPA can reach 3.9 g / L; the establishment of a whole cell transformation system solves the problems that the chemical synthesis of PPA is tedious in step and low in yield and results in environmental pollution and the enzymatic transformation production of PPA is low in transformation rate, realizes the pollution-free high-yield and one-step production of PPA, and lays a certain theoretical foundation for the subsequent industrial production.
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Description

technical field

[0001] The invention relates to a recombinant bacterium with improved transformation and production efficiency of α-phenylpyruvate, which belongs to the field of biotechnology. Background technique

[0002] α-Phenylpyruvate (PPA) has many applications. It can be used to synthesize heterocyclic compounds of antineoplastic drugs, as an antioxidant and to promote wound healing. It is the raw material for the synthesis of D-phenylalanine, which is a chiral drug intermediate. PPA can also be used to prepare phenyllactic acid, which can be used as an antibacterial substance. At present, the production of PPA is mainly chemical synthesis, mainly including: acetamidocinnamic acid hydrolysis method, hydantoin and benzaldehyde synthesis method and hydantoin method. These methods all require multi-step reactions and have high requirements for reaction conditions, such as high pressure, high temperature, etc., and the product yield is low, which increases the difficult...

Claims

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