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LDL-C (Low-Density Lipoprotein Cholesterol) quantitative detection method

A detection method and quantitative determination technology, applied in the biological field, can solve the problem of high price

Active Publication Date: 2015-08-26
王贤俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic clinical laboratories use homogeneous phase method to determine LDL-C reagents, which are mostly imported from Japan, such as the reagents produced by Ichika and Wako, or domestic reagents produced by foreign technologies, such as the products of Leadman and Shensuo. higher price

Method used

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  • LDL-C (Low-Density Lipoprotein Cholesterol) quantitative detection method
  • LDL-C (Low-Density Lipoprotein Cholesterol) quantitative detection method
  • LDL-C (Low-Density Lipoprotein Cholesterol) quantitative detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Preparation of reagent 1:

[0022]

[0023] 2. Preparation of reagent 2:

[0024]

[0025] Table 2 is the result data of the LDL-C detection kit prepared in Example 1, which was continuously measured for 10 times on the Hitachi 7180 automatic biochemical analyzer under the same external conditions.

[0026] Table 2 Accuracy measurement results table

[0027] Measurement times Result (mmol / L) 1 2.91 2 2.89

[0028] 3 3.00 4 2.98 5 2.85 6 2.92 7 2.78 8 2.88 9 2.94 10 2.88 average 2.90

[0029] As can be seen from Table 2, the accuracy of the LDL-C detection kit prepared in Example 1 is: 0.69%

[0030] Table 3 shows the result data of 20 consecutive determinations of quality control serum on a Hitachi 7180 automatic biochemical analyzer using the LDL-C detection kit prepared in Example 1 under the same external conditions.

[0031] Table 3 Precision measurement result table

[003...

Embodiment 2

[0040] 1. Preparation of reagent 1:

[0041]

[0042]

[0043] 2. Preparation of reagent 2:

[0044]

[0045] Table 2 is the result data of the LDL-C detection kit prepared in Example 2, which was continuously measured for 10 times on the Hitachi 7180 automatic biochemical analyzer under the same external conditions.

[0046] Table 2 Accuracy measurement results table

[0047] Measurement times Result (mmol / L) 1 2.58 2 2.64 3 2.56 4 2.57 5 2.62 6 2.53 7 2.56 8 2.59 9 2.59 10 2.62 average 2.59

[0048] As can be seen from Table 2, the accuracy of the LDL-C detection kit prepared in Example 2 is: 0.81%

[0049] Table 3 shows the result data of 20 consecutive determinations of quality control serum on the Hitachi 7180 automatic biochemical analyzer with the LDL-C detection kit prepared in Example 2 under the same external conditions.

[0050] Table 3 Precision measurement result table

[0051] ...

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Abstract

The invention discloses a detection method for quantitatively determining LDL-C (Low-Density Lipoprotein Cholesterol) by a joint covering method. Double liquid reagents are adopted and formed by a reagent 1 and a reagent 2 which are placed separately, wherein metal chloride, an LDL-C monoclonal antibody and a quantitative enzyme reagent are contained in the reagent 1 and a surface active agent, a color developing agent, a preservative, anti-interference materials and a reaction promoter are contained in the reagent 2. According to the LDL-C quantitative detection method, the color developing characteristic is good, the accuracy is high, the reagent composition is stable, the operation is simple, and the LDL-C quantitative detection method is suitable for various clinical full-automatic biochemical analyzers and is used for detecting the content of the LDL-C in serum of a human body.

Description

Technical field: [0001] The invention discloses a detection method for quantitatively measuring LDL-C through a combined masking method, which belongs to the field of biotechnology. Background technique: [0002] Low-density lipoprotein (LDL) is the most abundant lipoprotein in human blood, and the cholesterol (LDL-C) it carries is considered to be the main pathogenic factor of atherosclerosis and coronary heart disease, and clinical detection has become increasingly important. [0003] At present, there is no real reference method for measuring LDL-C. The CDC tentative reference method for measuring LDL-C is the ultracentrifugation method (Betaquantification, β-quantitative method / BQ method), which is also recommended by NCEP. The LDL-C measured by this method actually includes the cholesterol content of lipoprotein (a) [Lp(a)] and intermediate density lipoprotein (IDL), which is also the basis for evaluating the correctness of other detection methods. This method require...

Claims

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Application Information

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IPC IPC(8): G01N33/92G01N33/531
CPCG01N33/531G01N33/92G01N2800/323G01N2800/324
Inventor 王贤俊郭二豪郑蓓蕾江新涛
Owner 王贤俊
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