Targeted Nanophotopharmaceuticals for Cancer Photodynamic Therapy
A photodynamic therapy and targeting technology, which can be used in drug combination, drug delivery, preparations for in vivo experiments, etc., and can solve problems such as limiting the total dose of reactive oxygen species.
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Embodiment 1
[0099] Embodiment 1: with Ce 6 Preparation of Nanophotomedicine NPM-1 as Photosensitizer
[0100] In this example, the photosensitizer chlorin e 6 (Ce 6 ) of nanophotomedicine (i.e., NPM-1), the optical absorption of the final structure of the nanophotomedicine in the Q-band (654nm) region is free Ce 6 about 3 times.
[0101]Ce at a concentration of 1 μM in 5 ml of 99% DMSO 6 (commercially available from, eg, Porphyrin Products (Logan, Utah)) was reacted with a 10-15 fold molar excess of EDAC and a 10-15 molar excess of sulfo-NHS. After reacting for 4 hours, the combined product was purified by gel filtration to obtain an ammonia-reactive photosensitizer, which was further reacted with 200 μL of silane coupling agent APTS. The coupling reaction is carried out in the dark at room temperature for 3 to 4 hours to obtain the compound Ce 6 -APTS. In the next step, in 10 ml of 99% ethanol medium, Ce 6 - APTS was reacted with 600 μL (about 600 mg) of TEOS or TMOS for 2-3 ho...
Embodiment 2
[0103] Example 2: With Ce 6 Characteristics of Nanophotopharmaceutical NPM-2 as Photosensitizer
[0104] In this example, the ratio of Q-band light absorption to free Ce 6 Increased nanophotomedicine (NPM-2) process by about 4 times.
[0105] Ce at a concentration of 1 μM in 5 ml of 99% ethanol 6 React with 10-15 times molar excess of EDAC and 10-15 molar excess of sulfo-NHS. After about 4 hours of reaction, the conjugate was purified by gel filtration to obtain an ammonia-reactive photosensitizer, which was reacted with 300 μL of silane coupling agent APTS. The coupling reaction was carried out in the dark at room temperature for 3 to 4 hours to obtain the compound Ce 6 -APTS. In the next step, in 10 ml of 99% ethanol medium, Ce 6 -APTS was reacted with 800 μL of TEOS or TMOS for 3 hours to generate the precursor of NPM-2. Add 3 ml of water and 600 μL of NH 4 o 4 , at intervals of 2 minutes, ultrasonic treatment was performed for 15 minutes to hydrolyze the precurs...
Embodiment 3
[0106] Embodiment 3: with Ce 6 Preparation of Nanophotomedicine NPM-3 as Photosensitizer
[0107] In yet another embodiment, the absorption ratio in the Q-band region is illustrated for free Ce 6 Increased preparation of nanophotomedicine (NPM-3) by approximately 7-fold.
[0108] Ce at a concentration of 1 μM in 5 ml of 99% ethanol 6 React with 10-15 times molar excess of EDAC and 10-15 molar excess of sulfo-NHS. After 4 hours of reaction, the combined product was purified by gel filtration to obtain ammonia-reactive Ce 6 , ammonia-reactive Ce 6 React with 600 μL of silane coupling reagent APTS. The coupling reaction was carried out in the dark at room temperature for 3 to 4 hours to obtain the compound Ce 6 -APTS. In the next step, in 10 ml of 99% ethanol medium, Ce 6 -APTS was reacted with 1000 μL of TEOS or TMOS for 2-3 hours to form a precursor of silane-coupled quasi-aggregated photopharmaceuticals. Add 3ml of water and 800μL of NH 4 o 4 , sonicated for 20 mi...
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