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Method for performing sterilization by using mixed antibacterial agent in cultivation process of chlamydomonas reinhardtii

The technology of an antibacterial agent and reinhardtii is applied in the field of removing bacteria and fungi from Chlamydomonas reinhardtii, which can solve problems such as contamination of miscellaneous bacteria, and achieve the effects of strong killing ability, easy popularization and application, and good application prospect.

Active Publication Date: 2015-09-09
XUZHOU NORMAL UNIVERSITY
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for removing bacteria in the cultivation process of Chlamydomonas reinhardtii by using mixed antibacterial agents, so as to solve the problem of bacterial contamination in the experimental and application process of Chlamydomonas control

Method used

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  • Method for performing sterilization by using mixed antibacterial agent in cultivation process of chlamydomonas reinhardtii
  • Method for performing sterilization by using mixed antibacterial agent in cultivation process of chlamydomonas reinhardtii
  • Method for performing sterilization by using mixed antibacterial agent in cultivation process of chlamydomonas reinhardtii

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Embodiment 1

[0026] Embodiment 1: A. Inoculate the Chlamydomonas mixed bacteria contamination on the TAP solid medium plate, at 23 ± 0.5 ° C, 14 / 10 hours light and dark photoperiod, 8000Lx light intensity to cultivate for 3-5 days; B. Prepare a mixed antibacterial agent TAP solid medium plate containing 10ug / ml azoxystrobin and 15ug / ml nalidixic acid for use; On the TAP plate, culture at 23±0.5°C, 14 / 10 hour light and dark photoperiod, and 8000Lx light intensity for 5-8 days; D. Transfer the bacterium-eliminated Chlamydomonas to ordinary TAP solid again On the medium plate, culture at 23±0.5°C, 14 / 10 hour light and dark photoperiod, and 8000Lx light intensity for 3-5 days, for subsequent experiments or preservation for future use.

[0027] The specific steps are:

[0028] 1. Activation of contaminated Chlamydomonas: inoculate the contaminated Chlamydomonas on a TAP solid medium plate, and cultivate it for 3-5 days at 23±0.5°C, 14 / 10 hours light and dark photoperiod, and 8000Lx light inten...

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Abstract

The invention discloses a method for performing sterilization by using a mixed antibacterial agent in the cultivation process of chlamydomonas reinhardtii and belongs to a method for removing bacteria and fungi from chlamydomonas reinhardtii. The method comprises the following steps of: A. inoculating mixed infectious microbes polluted chlamydomonas on a TAP solid medium plate and cultivating for 3 to 5 days under the conditions of 23 + / - 0.5 DEG C, 14 / 10 hour light / dark period and light intensity of 8000 Lx; B. preparing a mixed antibacterial agent of azoxystrobin of 10 mu g / ml and nalidixic acid of 15 mu g / ml for later use on the TAP solid medium plate; C. transferring and lining the cultivated infectious microbe-containing chlamydomonas to the TAP plate containing the mixed antibacterial agent and cultivating for 5 to 8 days under the conditions of 23 + / - 0.5 DEG C, 14 / 10 hour light / dark period and light intensity of 8000 Lx; and D. transferring and lining the microbe-removed chlamydomonas to the common TAP solid medium plate again, cultivating for 3 to 5 days under the conditions of 23 + / - 0.5 DEG C, 14 / 10 hour light / dark period and light intensity of 8000 Lx, and performing subsequent experiments or performing conservation for standby application. The method has the advantage that fungi are removed by azoxystrobin and bacteria are removed by nalidixic acid, so that the problem about infectious microbe pollution in scientific experiments and application process of the chlamydomonas reinhardtii is solved.

Description

technical field [0001] The invention relates to a method for eliminating bacteria and fungi of Chlamydomonas reinhardtii, in particular to a method for eliminating bacteria in the cultivation process of Chlamydomonas reinhardtii by using mixed antibacterial agents. Background technique [0002] Chlamydomonas reinhardtii is a eukaryotic unicellular microalgae with short growth cycle, easy cultivation, annotated genome, mature related genetic biochemical technology and molecular biology technology, heavy metal adsorption capacity, high oil production, etc. As a model organism for the research and application of life sciences, the research on Chlamydomonas, a model of ciliated diseases, and the research on biodiesel and biodecontamination have been carried out. However, in the process of scientific research and application, it is inevitable to cause the pollution of Chlamydomonas species during inoculation, and these miscellaneous bacteria pollution will endanger the preservati...

Claims

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Application Information

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IPC IPC(8): C12N1/12A01N43/90A01P1/00A01P3/00A01N43/54
Inventor 王亮陆军郑元林陈慧怡杨丰源
Owner XUZHOU NORMAL UNIVERSITY
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