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Method for measuring content of index components in traditional Chinese medicine, palmleaf raspberry fruit

A determination method and technology for raspberries, which are applied in the directions of measuring devices, material separation, and analysis of materials, can solve problems such as the quality and quality of raspberries that are difficult to use in traditional Chinese medicine, and achieve the effects of shortening the total detection time, good repeatability, and saving reagent costs.

Active Publication Date: 2015-09-09
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to scientifically evaluate and control the quality of traditional Chinese medicine raspberry only through the identification of characters and powders.

Method used

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  • Method for measuring content of index components in traditional Chinese medicine, palmleaf raspberry fruit
  • Method for measuring content of index components in traditional Chinese medicine, palmleaf raspberry fruit
  • Method for measuring content of index components in traditional Chinese medicine, palmleaf raspberry fruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] (1) Preparation of test solution

[0063] Do the following treatments to the traditional Chinese medicine raspberries from 7 origins respectively:

[0064] Crush the traditional Chinese medicine raspberry, pass through a 40-mesh sieve, accurately weigh 0.5g, put it in a conical flask, accurately add 50mL of 70% methanol, seal it tightly, weigh 44.1g, ultrasonicate at room temperature for 30min, weigh again, and use 70% Make up the lost weight with aqueous methanol, shake well, centrifuge at 14,000 rpm for 10 min, and take 1 mL of the supernatant.

[0065] Each treatment was repeated 3 times.

[0066] (2) Preparation of reference substance solution

[0067] Accurately weigh 7.75mg of ellagic acid reference substance, add 100% methanol to dissolve and dilute to 25mL, and prepare 0.31mg·mL -1 Reference substance stock solution.

[0068] Precisely draw 1.5mL of ellagic acid reference substance stock solution, dilute to 5mL with 100% methanol, and prepare 93.00μg·mL -1 ...

Embodiment 2

[0103] (1) Preparation of test solution

[0104] With embodiment 1 step (1).

[0105] (2) Preparation of reference substance solution

[0106] Accurately weigh 7.75 mg and 8.18 mg of the ellagic acid reference substance and kaempferol-3-O-rutinoside reference substance respectively, add 100% methanol to dissolve them, and dilute to 25 mL and 10 mL respectively, and prepare 0.31 mg·mL respectively -1 Ellagic acid reference substance stock solution and 0.82mg·mL -1 Kaempferol-3-O-rutinoside reference substance stock solution.

[0107] Precisely draw 1.5mL and 0.2mL of ellagic acid reference substance stock solution and kaempferol-3-O-rutinoside reference substance stock solution respectively, mix them, and dilute to 5mL with 100% methanol to prepare 93.00μg·mL -1 Ellagic acid, 32.72μg·mL -1 Kaempferol-3-O-rutinoside mixed reference solution was diluted step by step with 1mL methanol solution with a volume fraction of 100%, and the concentrations of ellagic acid were 46.50, 2...

Embodiment 3

[0146] (1) Preparation of test solution

[0147] Do the following treatments to the traditional Chinese medicine raspberries from 7 origins respectively:

[0148] Crush raspberry, a traditional Chinese medicine, pass through a 40-mesh sieve, accurately weigh 1.0g, put it in a conical flask, accurately add 50mL of 70% methanol, seal it tightly, weigh 44.6g, ultrasonicate at room temperature for 30min, weigh, and use 70% methanol Make up the lost weight with aqueous solution, shake well, centrifuge at 14,000 rpm for 10 min, and take 1 mL of supernatant.

[0149] Each treatment was repeated 3 times.

[0150] (2) Preparation of reference substance solution

[0151] Accurately weighed adenosine, gallic acid, brevifolia hematoxylic acid, ethyl gallate, kaempferol-3-O-rutinoside, kaempferol-3-O-glucoside and lindenol reference substance 5.80mg, 6.90mg, 7.43mg, 6.89mg, 6.33mg, 7.65mg and 5.89mg, respectively add 100% methanol (containing a small amount of DMSO) to dissolve and set ...

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Abstract

The embodiment of the invention discloses a method for measuring the content of index components in a traditional Chinese medicine, palmleaf raspberry fruit. The method comprises the following steps: (1) preparing a test sample solution; (2) preparing a mixed contrast solution; (3) performing ultra high performance liquid chromatography detection; (4) constructing standard curves of N index components; and (5) determining the content of each of the N index components in a test sample. The method for measuring the content of the index components in the traditional Chinese medicine, palmleaf raspberry fruit, is accurate and efficient and can be used for controlling the quality of the traditional Chinese medicine, palmleaf raspberry fruit; furthermore, the method can be used for simultaneously detecting the various index components in the traditional Chinese medicine, palmleaf raspberry fruit; compared with an existing method for respectively detecting different index components, the method disclosed by the invention has the advantages that the total detection time is shortened, and the reagent cost is reduced; meanwhile, the detection result is accurate, the precision is high, the repetitiveness is high, the stability is high, and the specificity is excellent.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine quality control, in particular to a method for measuring the content of index components in the traditional Chinese medicine raspberry. Background technique [0002] The traditional Chinese medicine raspberry is the dried fruit of Rubus chingii Hu in the Rosaceae family. Harvest when the fruit turns from green to green-yellow in early summer, remove the stalks and leaves, put it in boiling water for a while or steam it, take it out, and dry it to get the traditional Chinese medicine raspberry. The traditional Chinese medicine raspberry has the effects of nourishing the kidney, consolidating essence and shrinking urine, nourishing the liver and improving eyesight. Modern pharmacological studies have shown that raspberry has antioxidant, anti-inflammatory, antithrombotic, and anticancer activities, and its chemical components mainly include flavonoids, terpenes, steroids, and phenolic ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 柴欣杨静杜龙飞江振作李洁朱彦王跃飞贺英
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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