Medicinal use of matsutake high-yielding strain (t35) in antioxidant drugs
A high-yielding strain and anti-oxidant technology, applied in the direction of drug combination, medical raw materials derived from fungi, anti-toxic agents, etc., can solve the problems of obstacles to the development of the matsutake industry, difficulties in artificial cultivation, etc., and achieve significant antioxidant effects, no toxic side effects, Simple and feasible method
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Embodiment 1
[0023] Preparation of matsutake high-yield strain (T35) fermentation liquid by submerged fermentation of matsutake high-yield strain (T35)
[0024] Put the culture solution of matsutake high-yield strain (T35) into 60L liquid fermentation medium according to the inoculum amount of 7% (glucose 20g / L, yeast extract powder 15g / L, KH2PO4 0.5g / L, MgSO7.H2O 0.5g / L, vitamin B1 0.2g / L), carry out submerged fermentation culture in a 100L fermenter at 26°C, 150r / min, ventilation rate 4L / min, initial pH 5.0, and culture for 48h to obtain the matsutake high-yield strain (T35) fermentation broth.
Embodiment 2
[0026] Preparation of matsutake high-yielding strain (T35) bacterial powder by vacuum freeze-drying
[0027] Centrifuge the fermentation broth of matsutake high-yielding strain (T35) at 4500 rpm for 5 minutes, discard the supernatant, keep the precipitate, and remove the water by vacuum freeze-drying technology. The wet bacteria powder was coated with a thickness of 10mm and pre-frozen at -26°C for 8 hours, and then vacuum freeze-dried, which was divided into four stages: the first stage was dried at -25°C for 10 hours, and the second stage was dried at -5°C for 10 hours. h, the third stage was dried at 15°C for 12 h, and the fourth stage was dried at 40°C for 8 h. The dried matsutake fungus was pulverized with a pulverizer and passed through a 300-mesh sieve to obtain matsutake high-yield strain (T35) fungus powder.
Embodiment 3
[0029] Preparation of matsutake high-yield strain (T35) bacterial powder by spray drying
[0030] Centrifuge the fermentation liquid of matsutake high-yielding strain (T35) at 4500 rpm for 5 minutes, discard the supernatant, keep the precipitate, and prepare the bacterial powder by spray drying technology. First, the matsutake fungus is homogenized, the homogenization temperature is 60° C., the homogenization pressure is 20 MPa, and after homogenization, it is passed through a 300-mesh filter cloth. Then spray dry matsutake fungus homogeneous liquid, the spray drying parameters are: air inlet temperature 150°C, hot air flow rate 36m3 / h, compressed air flow rate 490L / h, feed rate 4.0*180mL / h, air outlet temperature prediction The value is 60°C, and the pine fungus powder is collected at the outlet to obtain the matsutake matsutake high-yield strain (T35) fungus powder.
[0031] The following test shows the medical application of matsutake high-yield strain (T35) of the present...
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