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Willow needle mushroom glycocalyx, as well as preparation method and application thereof

A technology of Flammulina velutipes polysaccharides and complexes, which is applied in the fields of application, food preparation, food science, etc., can solve the problems of extraction and biological activity of Flammulina velutipes polysaccharides without willow, and achieve important economic value and market value

Active Publication Date: 2015-09-23
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no products related to the proteoglycan of Flammulina velutipes in the domestic and foreign markets, and there are no reports on the extraction and biological activity of polysaccharides from Flammulina velutipes in the literature

Method used

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  • Willow needle mushroom glycocalyx, as well as preparation method and application thereof
  • Willow needle mushroom glycocalyx, as well as preparation method and application thereof
  • Willow needle mushroom glycocalyx, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Fermentation: Inoculate Flammulina velutipes strains in the seed culture medium, the culture conditions are: temperature 26°C, rotation speed 120r / min, after cultivating for 5 days, insert the fermentation medium with 10% inoculation amount, the fermentation conditions are: temperature 26°C, rotation speed 120r / min, after culturing for 5 days, centrifuge at 4000r / min to collect the supernatant to obtain a crude polysaccharide-protein complex fermentation broth; the composition of the fermented seed medium of Flammulina velutipes polysaccharides is: 200g of potatoes; 15g of glucose; KH 2 PO 4 1.5g; MgSO 4 ·7H 2 O, 0.75g; V B1 , 1mg; water, 1000mL; fermentation medium composition: glucose, 100g; corn flour 200g; yeast extract powder, 60g; KH 2 PO 4 , 1.5g; MgSO 4 ·7H 2 O, 7.5g; V B1 , 10mg; water, 1000mL;

[0016] (2) Extraction: Concentrate the crude polysaccharide-protein complex fermentation liquid by rotary evaporation to 1 / 10 of the original body, add 3 t...

Embodiment 2

[0018] (1) Fermentation: Inoculate Flammulina velutipes strains in the seed culture medium, the culture conditions are: temperature 24°C, rotation speed 140r / min, after cultivating for 5.5 days, insert the fermentation culture medium with 15% inoculum amount, the fermentation conditions are: The temperature is 24°C, the rotational speed is 140r / min, and after culturing for 5 days, the supernatant is collected by centrifugation at 4000r / min to obtain a crude polysaccharide-protein complex fermentation broth; the composition of the willow flammulina velutipes polysaccharide fermentation seed medium is: potato 250g; glucose 17.5g; KH 2 PO 4 1.75g; MgSO 4 ·7H 2 O, 0.88g; V B1 , 1.25mg; water, 1000mL; fermentation medium composition: glucose, 150g; corn flour 300g; yeast extract powder, 60g; KH 2 PO 4 , 1.75g; MgSO 4 ·7H 2 O, 8.8g; V B1 , 12.5mg; Water, 1000mL;

[0019] (2) Extraction: Concentrate the crude polysaccharide-protein complex fermentation broth to 1 / 9 of the ori...

Embodiment 3

[0021] (1) Fermentation: Inoculate Flammulina velutipes strains in the seed medium, the culture conditions are: temperature 22°C, rotation speed 160r / min, after cultivating for 6 days, insert the fermentation medium with 20% inoculum, the fermentation conditions are: temperature 22°C, rotation speed 160r / min, after culturing for 6 days, centrifuge at 6000r / min to collect the supernatant to obtain the crude polysaccharide-protein complex fermentation broth; the composition of the willow flammulina velutipes polysaccharide fermentation seed medium is: 300g of potatoes; 20g of glucose; KH 2 PO 4 2.0g; MgSO 4 ·7H 2 O, 1g; V B1 , 1.5mg; water, 1000mL; fermentation medium composition: glucose, 200g; corn flour 400g; yeast extract powder, 60g; KH 2 PO 4 , 2.0g; MgSO 4 ·7H 2 O, 10g; V B1 , 15mg; water, 1000mL;

[0022] (2) Extraction: Concentrate the crude polysaccharide-protein complex fermentation broth to 1 / 8 of the original body by rotary evaporation, add 5 times the volum...

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Abstract

The invention relates to a willow needle mushroom glycocalyx, as well as a preparation method and application thereof, and belongs to the field of natural medicine development. The preparation method comprises the following steps: inoculating willow needle mushroom seeds into a culture medium, wherein the culture conditions such as temperature, rotational speed and time are 22 to 26 DEG C, 120 to 160 r / min, and 5 to 6 days respectively; inoculating 10 to 20 percent inoculum concentration of the willow needle mushroom seeds into a fermentation culture medium, wherein the fermentation conditions such as temperature, rotational speed and time are 22 to 26 DEG C, 120 to 160 r / min, and 5 to 6 days respectively; carrying out centrifugation to collect a supernatant at the rotational speed of 4000 r / min to 6000 r / min; carrying out rotary evaporation and concentration till the volume is 1 / 10 to 1 / 8 of the original volume; adding anhydrous ethanol of which the volume is 3 to 5 times of the concentrated liquid at the temperature of 4 DEG C for 12 to 16 hours' precipitation; carrying out 10 to 20 minutes' centrifugation, wherein the rotational speed is 3000 to 5000 r / min; collecting the precipitate; drying the precipitate at 45 to 50 DEG C. The willow needle mushroom glycocalyx fills up the blank of resource development and utilization of willow needle mushroom in the market. Moreover, an important foundation for deep development of polysaccharide immunological accelerator medicine and health foods with immunomodulatory effects is provided, and the economic value and the marketable value are high.

Description

technical field [0001] The invention belongs to the field of natural medicine development, and in particular relates to a fermented liquid extract of Flammulina velutipes and its preparation method and application. Background technique [0002] Edible and medicinal fungi are a very important class of natural biological resources, and have become an important research object in the discovery of new drugs and the development of functional foods. Studies have shown that edible and medicinal fungi contain polysaccharides, proteins, flavonoids and other active substances, which have various pharmacological effects such as improving immunity, anti-tumor, and anti-oxidation. At present, Lentinan, Ganoderma lucidum polysaccharide, and Polyporus polysaccharide have been approved by the health department and have become clinical anti-tumor immunotherapy drugs. Although a large number of edible and medicinal fungus polysaccharides have been developed and utilized, due to the rich fung...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/06A61P37/02A61P35/00A23L1/29
Inventor 王琦朱宴妍刘洋魏巍朱相杨王瑞
Owner JILIN AGRICULTURAL UNIV
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