Pseudomonas cepacia strain QBA-3 having function of inhibiting botrytis cinerea and application thereof

A technology for Pseudomonas cepacia and Botrytis cinerea, applied in the field of microorganisms, can solve problems such as affecting the sustainable and healthy development of agriculture, excessive pesticide residues, environmental pollution, etc., and achieve the effect of good market application prospects.

Inactive Publication Date: 2015-09-23
QINGDAO AGRI UNIV
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Problems solved by technology

[0003] Botrytis cinerea reproduces very fast and produces a large number of conidia, so it is easy to mutate and produce drug resistance. At present, it has been used in agricultural production for benzimazoles, dicarboximides, and carbamates. Resistance to various fungicides such as fungicides, which not only leads to the reduction of control efficie

Method used

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  • Pseudomonas cepacia strain QBA-3 having function of inhibiting botrytis cinerea and application thereof
  • Pseudomonas cepacia strain QBA-3 having function of inhibiting botrytis cinerea and application thereof
  • Pseudomonas cepacia strain QBA-3 having function of inhibiting botrytis cinerea and application thereof

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Embodiment 1

[0020] 1. Isolation and screening of strains

[0021] On March 19, 2013, 20 tomato leaf samples were collected in a greenhouse in Laiyang City. Each leaf was soaked in 75% alcohol for 10 seconds, then soaked in 0.1% mercury chloride for 30 seconds, rinsed with sterile water for 3 times, and transferred to sterilized water. Grind in a mortar treated with bacteria, add sterile water to dilute to 10ml, continue to dilute to 10ml with sterile water -5 times, take 100ul respectively and apply LB plates (tryptone 10 g / L, yeast extract 5 g / L, sodium chloride 5 g / L, pH 7.0), and culture at 30°C.

[0022] Pick a single colony and inoculate it in YEPD medium (yeast extract 3 g / L, peptone 10 g / L, glucose 20 g / L) at 25°C and 120 rpm for 24 hours, then culture at 25°C and 12000 rpm Centrifuge under the conditions, filter the supernatant through a filter with a diameter of 0.22um, inoculate the conidia of Botrytis cinerea into the filtrate and let it stand for cultivation, inoculate the co...

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Abstract

The invention discloses a pseudomonas cepacia strain QBA-3 having the function of inhibiting botrytis cinerea and an application thereof. The pseudomonas cepacia strain QBA-3 is obtained through screening, the class name of the QBA-3 is Pseudomonas cepacia, the QBA-3 is preserved in China Center for Type Culture Collection, and the preservation serial number of the QBA-3 is CCTCC M2015402. A botrytis cinerea spore germination experiment, a germinal tube elongation experiment and detached leaf experiment prove that the pseudomonas cepacia strain QBA-3 has the strong inhibiting function on the botrytis cinerea, the pseudomonas cepacia strain QBA-3 can be made into a biocontrol agent preventing the botrytis cinerea and has good market application prospects.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a Pseudomonas cepacia strain QBA-3 capable of inhibiting Botrytis cinerea and its application. Background technique [0002] Botrytis cinerea is an important plant pathogenic fungus widely distributed in the world. There are many kinds of hosts of this fungus, which can infect the stems, leaves, flowers and fruits of 235 kinds of plants, such as tomatoes and cucumbers, and grapes and strawberries. mildew. The disease can occur not only during the growing season of the host plant, but also during the storage of agricultural products, so the damage is serious. Especially in recent years, the large-scale promotion of protected cultivation methods has led to wide sources of infection and rapid spread of the fungus. Botrytis cinerea is becoming more and more serious, and its occurrence and prevalence often cause serious economic losses. According to reports, the occurre...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/38
CPCA01N63/10C12N1/205C12R2001/38
Inventor 周善跃高攀梁文星李保华李宝笃
Owner QINGDAO AGRI UNIV
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